Recombinant CDC16 抗体
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北京 101111
Quick Overview for Recombinant CDC16 抗体 (ABIN7880377)
抗原
See all CDC16 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- CDC16 Antibody / Cell division cycle protein 16
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纯化方法
- Affinity-chromatography
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免疫原
- A synthesized peptide derived from human Apc6 / CDC16 was used as the immunogen for the CDC16 antibody.
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亚型
- IgG
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anti-Cell Division Cycle 16 Homolog (S. Cerevisiae) (CDC16) (Internal Region) antibody
CDC16 适用: 人, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 16 Homolog (S. Cerevisiae) (CDC16) antibodyKD Validated CDC16 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB3455 unconjugated Recombinant Antibody
anti-Cell Division Cycle 16 Homolog (S. Cerevisiae) (CDC16) antibodyKD Validated CDC16 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB3415 unconjugated Recombinant Antibody
anti-Cell Division Cycle 16 Homolog (S. Cerevisiae) (CDC16) (AA 371-620) antibodyCDC16 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 16 Homolog (S. Cerevisiae) (CDC16) (AA 526-575), (pSer560) antibodyCDC16 适用: 人, 小鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 16 Homolog (S. Cerevisiae) (CDC16) (C-Term) antibodyCDC16 适用: 人, Pig, 猴 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 16 Homolog (S. Cerevisiae) (CDC16) (AA 371-620) antibodyCDC16 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 16 Homolog (S. Cerevisiae) (CDC16) (C-Term) antibodyCDC16 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the CDC16 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store the CDC16 antibody at -20oC.
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- CDC16 (Cell Division Cycle 16 Homolog (S. Cerevisiae) (CDC16))
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别名
- CDC16
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背景
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CDC16 antibody detects cell division cycle protein 16, a core component of the anaphase-promoting complex/cyclosome (APC/C). CDC16 belongs to the tetratricopeptide repeat (TPR) family of proteins, characterized by structural motifs that mediate protein-protein interactions. Within the APC/C, CDC16 functions as a scaffolding subunit that contributes to complex assembly, stability, and substrate recognition. The APC/C is a multi-subunit E3 ubiquitin ligase that controls progression through mitosis by targeting key regulators such as securin and cyclins for ubiquitin-dependent degradation.
Research using CDC16 antibody has demonstrated the protein's critical role in cell cycle transitions. During metaphase-to-anaphase progression, the APC/C promotes chromosome segregation by degrading securin, releasing separase to cleave cohesin complexes. Later, degradation of mitotic cyclins ensures exit from mitosis and resetting of the cell cycle. CDC16, in concert with CDC27 and CDC23, forms a TPR subcomplex essential for binding co-activators such as CDC20 and CDH1, which provide substrate specificity.
CDC16 has been implicated in oncogenesis and developmental biology. Dysregulation of APC/C function leads to chromosomal instability, aneuploidy, and uncontrolled proliferation, all hallmarks of cancer. Elevated CDC16 expression has been reported in certain tumors, where it may contribute to aberrant APC/C activity. Conversely, insufficient CDC16 function impairs APC/C activity, causing mitotic arrest and apoptosis. In developmental systems, proper APC/C activity ensures correct cell division during embryogenesis and tissue differentiation.
Functional studies in model organisms highlight the conserved nature of CDC16. Orthologs in yeast, flies, and mammals show similar roles in APC/C regulation, underscoring evolutionary conservation. Conditional knockout studies in mice reveal embryonic lethality when CDC16 function is disrupted, confirming its essential role in proliferation.
Antibodies against CDC16 are validated for western blot, immunoprecipitation, immunohistochemistry, and immunofluorescence. Researchers use these reagents to analyze CDC16 expression, monitor APC/C complex integrity, and study ubiquitination dynamics in dividing cells. Clone-based antibodies provide reproducibility, distinguishing CDC16 from other TPR-containing proteins.
NSJ Bioreagents supplies this CDC16 antibody to support research in cell cycle regulation, cancer biology, and ubiquitin-proteasome system function. -
UniProt
- Q13042
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途径
- Regulation of Actin Filament Polymerization
抗原
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