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CD45RB 抗体

The 小鼠 单克隆 anti-CD45RB antibody (Clone SMP569) (ABIN7880320) specifically detects CD45RB in WB 和 IHC (p). The antibody is reactive with 人 samples.
产品编号 ABIN7880320
发货至: 中国
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Quick Overview for CD45RB 抗体 (ABIN7880320)

抗原

See all CD45RB 抗体
CD45RB

适用

  • 97
  • 19
  • 10
  • 6
  • 5
  • 4
  • 3
  • 2

宿主

  • 68
  • 31
  • 19
小鼠

克隆类型

  • 105
  • 12
  • 1
单克隆

标记

  • 43
  • 10
  • 8
  • 7
  • 6
  • 6
  • 6
  • 6
  • 6
  • 6
  • 6
  • 5
  • 1
  • 1
  • 1
This CD45RB antibody is un-conjugated

应用范围

  • 86
  • 66
  • 60
  • 44
  • 32
  • 32
  • 21
  • 7
  • 5
  • 2
  • 1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

质量等级

Carrier-free

克隆位点

SMP569
  • 原理

    CD45RB Antibody (azide and preservative free)

    纯化方法

    Protein G affinity chromatography

    免疫原

    Human peripheral blood lymphocytes maintained in T cell growth factor was used as immunogen for this CD45RB antibody.

    亚型

    IgG1, kappa
  • 应用备注

    Due to variation in protocol and secondary antibody used, the CD45RB antibody may need to be titered for optimal performance.

    1. FFPE staining requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    The CD45RB antibody (with azide) can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.
  • 抗原

    CD45RB

    别名

    CD45RB

    背景

    CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45RB is expressed on mature B-lymphocytes and the majority of lymphomas and leukemias of B-cell origin. This antigen is also expressed on the surface of cells of myeloid lineage.

    UniProt

    P08575
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