Recombinant Biotin 抗体
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北京 101111
Quick Overview for Recombinant Biotin 抗体 (ABIN7880080)
抗原
抗体类型
宿主
克隆类型
标记
应用范围
质量等级
克隆位点
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原理
- Recombinant Biotin Antibody / Rabbit Monoclonal (azide and preservative free)
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纯化方法
- Protein A affinity chromatography
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免疫原
- Biotinylated sheep immunoglobulin was used as the immunogen for this recombinant Biotin antibody.
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亚型
- IgG, kappa
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应用备注
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The optimal dilution of the recombinant Biotin antibody for each application should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the recombinant Biotin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- Biotin
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物质类
- Chemical
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背景
- This mAb recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
抗原
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