Recombinant Actin alpha (ACTAlpha) 抗体
The 兔 单克隆 anti-Actin alpha (ACTAlpha) antibody (Clone CAD-1) (ABIN7879867) specifically detects Actin alpha (ACTAlpha) in WB, IHC (p) 和 IF.
The antibody is reactive with 人, 小鼠 和 大鼠 samples.
产品编号 ABIN7879867
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Quick Overview for Recombinant Actin alpha (ACTAlpha) 抗体 (ABIN7879867)
抗原
Actin alpha (ACTAlpha)
抗体类型
Recombinant Antibody
适用
人, 小鼠, 大鼠
宿主
兔
克隆类型
单克隆
标记
非结合性
应用范围
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
克隆位点
CAD-1
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原理
- Alpha Actin Antibody / ACTA2 / Smooth Muscle Actin
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纯化方法
- Affinity purified
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免疫原
- A synthetic peptide specific to human ACTA2 protein was used as the immunogen for the Alpha Actin antibody.
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亚型
- IgG
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应用备注
- Optimal dilution of the Alpha Actin antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Antibody in PBS with 0.02 % sodium azide, 50 % glycerol and 0.4-0.5 mg/mL BSA
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store the Alpha Actin antibody at -20oC.
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- Actin alpha (ACTAlpha)
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别名
- Alpha Actin
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背景
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Alpha Actin antibody is a key reagent for studying cytoskeletal dynamics, smooth muscle physiology, and tissue remodeling. The encoded protein, ACTA2 (actin alpha 2), is a smooth muscle specific isoform of actin that contributes to contraction, structure, and motility of vascular and visceral smooth muscle cells. As a fundamental component of the cytoskeleton, ACTA2 polymerizes into filaments that provide mechanical strength and coordinate intracellular signaling.
Alpha actin is highly expressed in the smooth muscle layers of blood vessels, the respiratory tract, the gastrointestinal system, and reproductive organs. It also serves as a well established marker for myofibroblasts, which emerge during wound healing and fibrotic disease. Upregulation of ACTA2 in fibroblasts indicates their transition into contractile myofibroblasts, a process central to tissue repair and pathologic fibrosis. Detection with Alpha Actin antibody allows researchers to track these changes in both normal physiology and disease progression.
In cardiovascular research, ACTA2 is examined as a marker of vascular smooth muscle cells and their response to injury or remodeling. Phenotypic switching of smooth muscle cells, from a contractile to a synthetic state, is associated with vascular disease such as atherosclerosis. In liver, kidney, and lung fibrosis, ACTA2 positive myofibroblasts accumulate and contribute to excessive extracellular matrix deposition. Because of this, the Alpha Actin antibody is routinely applied in histological studies of fibrotic disorders.
At the molecular level, ACTA2 shares high sequence similarity with other actin isoforms but contains unique features that confer smooth muscle specific function. It interacts with actin binding proteins to regulate filament turnover, contractility, and cell migration. The strong conservation of actin family proteins ensures that Alpha Actin antibody provides reliable detection across species in research models.
The Alpha Actin antibody is widely used in immunohistochemistry, immunofluorescence, western blotting, and flow cytometry. These applications enable localization and quantification of ACTA2 in tissue samples and cultured cells. For researchers focused on vascular biology, tissue fibrosis, or cytoskeletal organization, the Alpha Actin antibody is a specific and dependable detection tool. NSJ Bioreagents offers validated antibodies to ensure reproducibility and accuracy in molecular and cellular studies. -
UniProt
- P62736
抗原
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