Recombinant PBK 抗体 (pThr9)
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北京 101111
Quick Overview for Recombinant PBK 抗体 (pThr9) (ABIN7879809)
抗原
See all PBK 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- pThr9
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原理
- Phospho-PBK (Thr9) Antibody / PDZ binding kinase
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纯化方法
- Affinity-chromatography
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免疫原
- A synthesized peptide derived from human Phospho-PBK/TOPK (Thr9) was used as the immunogen for the Phospho-PBK (Thr9) Antibody.
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亚型
- IgG
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anti-PDZ Binding Kinase (PBK) antibody
PBK 适用: 人 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 2C8 unconjugated
anti-PDZ Binding Kinase (PBK) (AA 1-110) antibodyPBK 适用: 人 WB, ELISA, IHC (p), IF 宿主: 小鼠 Monoclonal 3A11 unconjugated
anti-PDZ Binding Kinase (PBK) (AA 58-86), (N-Term) antibodyPBK 适用: 人, 小鼠 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB40570 unconjugated
anti-PDZ Binding Kinase (PBK) (AA 1-322) antibodyPBK 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2D6 unconjugated
anti-PDZ Binding Kinase (PBK) (AA 1-110) antibodyPBK 适用: 人 WB, ELISA, IHC (p), IF 宿主: 小鼠 Monoclonal 4A10 unconjugated
anti-PDZ Binding Kinase (PBK) (AA 1-322) antibodyPBK 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-PDZ Binding Kinase (PBK) (pThr9) antibodyKD Validated PBK 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB4910 unconjugated Recombinant Antibody
anti-PDZ Binding Kinase (PBK) (AA 71-322) antibodyPBK 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-PDZ Binding Kinase (PBK) (AA 122-229) antibodyPBK 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2A3 unconjugated
anti-PDZ Binding Kinase (PBK) (AA 251-322) antibodyPBK 适用: 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the Phospho-PBK (Thr9) Antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store the Phospho-PBK (Thr9) Antibody at -20oC.
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- PBK (PDZ Binding Kinase (PBK))
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别名
- PBK
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背景
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Phospho-PBK (Thr9) antibody detects PDZ binding kinase, also known as lymphokine activated killer T cell origin protein kinase (TOPK), when phosphorylated at threonine 9. PBK is encoded by the PBK gene and belongs to the MAP kinase kinase family. It is highly expressed in proliferating cells, especially in testis, placenta, and activated lymphocytes. Phosphorylation at Thr9 regulates PBK kinase activity, influencing cell cycle progression and mitotic events. This makes Phospho-PBK (Thr9) antibody a key tool for studying proliferative signaling and tumor biology.
Phospho-PBK (Thr9) antibody is widely applied in oncology, immunology, and cell cycle research. PBK activation supports G2/M transition by phosphorylating downstream substrates involved in spindle formation and chromosome segregation. It also participates in immune signaling pathways in T cells and NK cells. By detecting phosphorylation at Thr9, researchers can monitor PBK activation and its role in proliferation and immunity.
Applications for Phospho-PBK (Thr9) antibody include western blotting, immunohistochemistry, and immunofluorescence. Western blot assays detect phosphorylated PBK isoforms, immunohistochemistry maps expression in tumors, and immunofluorescence reveals subcellular localization at centrosomes during mitosis. These complementary approaches provide detailed evaluation of PBK biology.
Dysregulated PBK activity is implicated in multiple cancers, including lung, breast, and hematologic malignancies. Elevated Thr9 phosphorylation correlates with tumor grade and poor prognosis, reflecting hyperactive proliferation. In immune biology, PBK phosphorylation regulates lymphocyte activation, linking cell cycle control to host defense. By applying Phospho-PBK (Thr9) antibody, scientists can study these dual roles in cancer and immunity.
Therapeutic research has targeted PBK as a potential kinase for inhibition. Small molecule inhibitors reduce proliferation and sensitize tumor cells to chemotherapy. Monitoring Thr9 phosphorylation provides a pharmacodynamic readout of drug activity. Phospho-PBK (Thr9) antibody from NSJ Bioreagents offers strong specificity for this site specific modification, supporting mechanistic and translational research into kinase biology. -
UniProt
- Q96KB5
抗原
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