Recombinant TBC1D4 抗体 (pThr642)
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Quick Overview for Recombinant TBC1D4 抗体 (pThr642) (ABIN7879804)
抗原
See all TBC1D4 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- pThr642
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原理
- Phospho-AS160 (Thr642) Antibody / TBC1D4
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纯化方法
- Affinity-chromatography
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免疫原
- A synthesized peptide derived from human Phospho-AS160 (T642) was used as the immunogen for the Phospho-AS160 (Thr642) antibody.
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亚型
- IgG
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anti-TBC1 Domain Family, Member 4 (TBC1D4) (AA 574-712) antibody
TBC1D4 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 8A11A2 unconjugated
anti-TBC1 Domain Family, Member 4 (TBC1D4) (AA 1-94) antibodyTBC1D4 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-TBC1 Domain Family, Member 4 (TBC1D4) (AA 172-201), (N-Term) antibodyTBC1D4 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB21034 unconjugated
anti-TBC1 Domain Family, Member 4 (TBC1D4) (Internal Region) antibodyVerified TBC1D4 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-TBC1 Domain Family, Member 4 (TBC1D4) antibodyTBC1D4 适用: 人 WB, FACS 宿主: 兔 Monoclonal 25GB890 unconjugated Recombinant Antibody
anti-TBC1 Domain Family, Member 4 (TBC1D4) antibodyTBC1D4 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-TBC1 Domain Family, Member 4 (TBC1D4) (AA 1118-1298) antibodyTBC1D4 适用: 人 WB, ELISA, IHC, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-TBC1 Domain Family, Member 4 (TBC1D4) (pThr642) antibodyTBC1D4 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-TBC1 Domain Family, Member 4 (TBC1D4) (AA 1155-1204) antibodyTBC1D4 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-TBC1 Domain Family, Member 4 (TBC1D4) (AA 200-250) antibodyTBC1D4 适用: 人 WB, IP, IHC (fp) 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the Phospho-AS160 (Thr642) antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store the Phospho-AS160 (Thr642) antibody at -20oC.
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- TBC1D4 (TBC1 Domain Family, Member 4 (TBC1D4))
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别名
- AS160
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背景
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Phospho-AS160 (Thr642) antibody detects TBC1 domain family member 4 when phosphorylated at threonine 642. This protein is encoded by the TBC1D4 gene and is commonly referred to as AS160, Akt substrate of 160 kDa, and insulin-regulated Rab GTPase-activating protein. TBC1D4 functions as a Rab GAP that regulates trafficking of GLUT4 vesicles in response to insulin signaling. Phosphorylation at Thr642 by Akt relieves its inhibitory GAP activity, allowing Rab GTPases to promote translocation of GLUT4 to the plasma membrane, a critical event in glucose uptake by adipocytes and muscle cells.
Phospho-AS160 (Thr642) antibody is widely applied in metabolism, endocrinology, and diabetes research. Detecting phosphorylation at Thr642 provides a direct readout of insulin-stimulated Akt signaling and GLUT4 trafficking. This makes the antibody valuable for studies of insulin resistance, obesity, and type 2 diabetes. In addition, TBC1D4 is expressed in other tissues, linking it to energy homeostasis beyond classical insulin-responsive cells.
Applications for Phospho-AS160 (Thr642) antibody include western blotting, immunohistochemistry, immunofluorescence, and ELISA. Western blot assays detect phosphorylation changes in response to insulin or exercise, immunohistochemistry maps expression in metabolic tissues, and immunofluorescence highlights vesicle trafficking events. These methods allow researchers to connect molecular signaling to physiological glucose uptake.
Dysregulation of TBC1D4 phosphorylation is linked to insulin resistance and diabetes. Reduced phosphorylation impairs GLUT4 trafficking, while mutations in TBC1D4 cause severe postprandial hyperinsulinemia and muscle glycogen storage abnormalities. By applying Phospho-AS160 (Thr642) antibody, scientists can study both common and rare metabolic disorders.
Beyond metabolism, TBC1D4 regulates vesicle trafficking in immune cells, neurons, and other systems. Phosphorylation-dependent regulation highlights its integration with Akt signaling in multiple contexts. NSJ Bioreagents provides Phospho-AS160 (Thr642) antibody with validated specificity, ensuring accurate detection of this phosphorylation event in insulin and trafficking research. -
UniProt
- O60343
抗原
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