Recombinant Phospholamban 抗体 (pSer16)
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Quick Overview for Recombinant Phospholamban 抗体 (pSer16) (ABIN7879712)
抗原
See all Phospholamban (PLN) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- pSer16
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原理
- Phospho-Phospholamban (S16) Antibody / PLN
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纯化方法
- Affinity-chromatography
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免疫原
- A synthesized peptide derived from human Phospho-Phospholamban (S16) was used as the immunogen for the Phospho-Phospholamban (S16) antibody.
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亚型
- IgG
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anti-Phospholamban (PLN) (AA 1-52) antibody
PLN 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Phospholamban (PLN) (AA 1-30) antibodyPLN 适用: 人 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Phospholamban (PLN) (pSer16) antibodyPLN 适用: 人 ELISA, IF (cc), IF (p), ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Phospholamban (PLN) (N-Term) antibodyVerified PLN 适用: 人, 大鼠 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Phospholamban (PLN) (AA 1-22), (N-Term) antibodyPLN 适用: 人 WB 宿主: 兔 Polyclonal RB44799 unconjugated
anti-Phospholamban (PLN) (pThr17) antibodyPLN 适用: 人, 大鼠 WB, DB 宿主: 兔 Polyclonal RB24137 unconjugated
anti-Phospholamban (PLN) (AA 1-35), (N-Term) antibodyPLN 适用: 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Phospholamban (PLN) (Thr17) antibodyPLN 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-Phospholamban (PLN) (AA 1-35) antibodyPLN 适用: 人 ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Phospholamban (PLN) (AA 1-30) antibodyPLN 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
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应用备注
- Optimal dilution of the Phospho-Phospholamban (S16) antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store the Phospho-Phospholamban (S16) antibody at -20oC.
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- Phospholamban (PLN)
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别名
- Phospholamban
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背景
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Phospho-Phospholamban (S16) antibody detects PHOSPHOLAMBAN, encoded by the PLN gene, when phosphorylated at serine 16. Alternate names include cardiac phospholamban, PLB, and phospholamban phosphoprotein. PHOSPHOLAMBAN is a small transmembrane protein in the sarcoplasmic reticulum of cardiac and skeletal muscle. It regulates SERCA2a, the sarcoplasmic reticulum Ca2+ ATPase responsible for calcium uptake during muscle relaxation. In its unphosphorylated state, PHOSPHOLAMBAN inhibits SERCA2a. Phosphorylation at serine 16 by PKA relieves this inhibition, enhancing calcium uptake, promoting relaxation, and increasing cardiac contractility.
Phospho-Phospholamban (S16) antibody is widely applied in cardiovascular biology, muscle physiology, and signaling research. By detecting phosphorylation at serine 16, researchers can study how beta adrenergic signaling enhances cardiac output through PKA mediated regulation of calcium cycling. This site specific antibody allows distinction between active and inhibited PHOSPHOLAMBAN, providing insights into heart function under physiological and pathological conditions.
Applications include western blotting, immunohistochemistry, immunofluorescence, and ELISA. Western blot assays detect phosphorylated PHOSPHOLAMBAN in heart lysates, immunohistochemistry maps phosphorylation in cardiac tissue sections, and immunofluorescence highlights localization in sarcoplasmic reticulum. ELISA assays allow quantification of phosphorylation status in experimental models. These techniques provide direct evidence of cardiac signaling events.
Dysregulation of PHOSPHOLAMBAN phosphorylation contributes to heart failure, arrhythmias, and cardiomyopathies. Mutations in PLN cause inherited dilated cardiomyopathy and arrhythmogenic right ventricular cardiomyopathy. Monitoring serine 16 phosphorylation provides biomarkers for disease progression and therapy efficacy. By applying Phospho-Phospholamban (S16) antibody, scientists can investigate how altered signaling leads to impaired cardiac relaxation and contractility.
Phosphorylation of PHOSPHOLAMBAN is also studied in exercise physiology, where beta adrenergic stimulation enhances contractility. The antibody supports research into how training, drugs, and genetic variants influence calcium cycling. Translational applications include evaluation of therapeutic interventions targeting SERCA2a regulation, such as gene therapy or pharmacologic modulators. NSJ Bioreagents offers Phospho-Phospholamban (S16) antibody with validated specificity, supporting robust research into cardiac physiology and disease. -
UniProt
- P26678
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途径
- Negative Regulation of Transporter Activity
抗原
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