Recombinant PKC 抗体 (pan)
Our Local Distributor
北京 101111
Quick Overview for Recombinant PKC 抗体 (pan) (ABIN7879698)
抗原
See all PKC (PKC pan) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- pan
-
原理
- Pan-PKC Antibody / PRKCA/PRKCB/PRKCD/PRKCE/PRKCG/PRKCZ
-
纯化方法
- Affinity chromatography
-
免疫原
- A synthesized peptide derived from human PKC was used as the immunogen for the Pan-PKC antibody.
-
亚型
- IgG
-
-
anti-Protein Kinase C (pan) (PKC pan) (pThr497) antibody
PKC pan 适用: 人, 大鼠, 小鼠 WB, IF, IHC, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C (pan) (PKC pan) (AA 623-672) antibodyPKC pan 适用: 人, 大鼠, 小鼠 WB, IF, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C (pan) (PKC pan) (pSer660) antibodyPKC pan 适用: 人 WB, FACS 宿主: 兔 Monoclonal 24GB5450 unconjugated Recombinant Antibody
anti-Protein Kinase C (pan) (PKC pan) (Thr497) antibodyPKC pan 适用: 人, 大鼠, 小鼠 WB, IF, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase C (pan) (PKC pan) (pThr497) antibodyPKC pan 适用: 人, 大鼠, 小鼠 WB, IF, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- Optimal dilution of the Pan-PKC antibody should be determined by the researcher.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C
-
储存方法
- Store the Pan-PKC antibody at -20oC.
-
-
- PKC (PKC pan) (Protein Kinase C (pan) (PKC pan))
-
别名
- PKC
-
背景
-
Pan-PKC antibody detects multiple isoforms of Protein kinase C, including PRKCA, PRKCB, PRKCD, PRKCE, PRKCG, and PRKCZ. Protein kinase C represents a family of serine threonine kinases that play central roles in transducing signals from membrane receptors to diverse downstream pathways. These kinases regulate processes such as cell growth, differentiation, apoptosis, immune responses, and neurotransmission. Pan-PKC antibody provides a broad detection tool for studying Protein kinase C activity across its isoforms, enabling researchers to assess general pathway activation in cellular models and tissues.
The Protein kinase C family is divided into conventional, novel, and atypical classes based on their structural domains and activation requirements. Conventional isoforms such as PRKCA, PRKCB, and PRKCG require calcium and diacylglycerol for activation, while novel isoforms including PRKCD and PRKCE are calcium independent but still activated by diacylglycerol. Atypical isoforms such as PRKCZ do not respond to either calcium or diacylglycerol but are regulated by other lipid derived second messengers. Research using Pan-PKC antibody has shown that this kinase family integrates multiple extracellular signals, allowing cells to adapt rapidly to changing environments.
Aberrant Protein kinase C signaling is implicated in cancer, diabetes, cardiovascular disease, and neurological disorders. Overexpression or mutation of individual isoforms alters cell cycle progression, apoptosis thresholds, and stress responses. For example, PRKCA is frequently upregulated in breast cancer, while PRKCD contributes to apoptosis in lymphoid cells. Using Pan-PKC antibody allows researchers to detect total family expression or activation states, providing a comprehensive readout when isoform specificity is not required. This broad recognition is especially valuable in screening studies where pathway activity is assessed across multiple tissues and disease contexts.
Pan-PKC antibody is widely applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting enables detection of multiple isoforms in complex lysates, while immunohistochemistry highlights expression patterns across tissues such as brain, liver, and immune organs. Immunofluorescence demonstrates localization of Protein kinase C to membranes, cytoplasm, or nucleus depending on isoform activation state. Functional studies with Pan-PKC antibody reveal dynamic translocation patterns following receptor stimulation, underscoring the central role of this kinase family in signaling.
By supplying validated Pan-PKC antibody reagents, NSJ Bioreagents supports research into cell signaling, disease mechanisms, and therapeutic development. Detection of multiple Protein kinase C isoforms with a single reagent ensures broad coverage of this essential kinase family and facilitates studies where general PKC activity is the focus. -
UniProt
- P05129
-
途径
- Positive Regulation of Response to DNA Damage Stimulus
抗原
-
