ODF1 抗体 (N-Term)
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Quick Overview for ODF1 抗体 (N-Term) (ABIN7879639)
抗原
See all ODF1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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原理
- ODF1 Antibody / Outer dense fiber protein 1
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纯化方法
- Immunogen affinity purified
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human ODF1 was used as the immunogen for the ODF1 antibody.
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亚型
- IgG
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anti-Outer Dense Fiber of Sperm Tails 1 (ODF1) (AA 117-166) antibody
ODF1 适用: 人, 小鼠, 大鼠, Cow, 犬, 山羊, 豚鼠, 马, 兔, Bat, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Outer Dense Fiber of Sperm Tails 1 (ODF1) (AA 80-180) antibodyODF1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Outer Dense Fiber of Sperm Tails 1 (ODF1) (Middle Region) antibodyODF1 适用: 人, 小鼠, 大鼠, Cow, 犬, 山羊, 豚鼠, 马, 兔 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the ODF1 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the ODF1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- ODF1 (Outer Dense Fiber of Sperm Tails 1 (ODF1))
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别名
- ODF1
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背景
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ODF1 antibody detects Outer dense fiber protein 1, encoded by the ODF1 gene on chromosome 8q22.2. ODF1 antibody is widely applied in reproductive biology, spermatogenesis, and fertility research. ODF1 is a structural protein of the sperm tail, specifically localized to the outer dense fibers that run alongside the axoneme in the principal piece of the flagellum. These fibers provide mechanical strength, protect the axoneme from shear stress, and contribute to the elastic recoil properties of sperm movement.
Structurally, ODF1 is a ~27 kDa coiled-coil protein with leucine zipper motifs that promote oligomerization. It is one of the earliest proteins incorporated into outer dense fibers during spermatid differentiation. ODF1 interacts with other outer dense fiber proteins such as ODF2 and with axonemal microtubules, forming the cytoskeletal backbone of the sperm tail.
Functionally, ODF1 contributes to sperm motility by reinforcing the axoneme and supporting energy transmission along the flagellum. Loss of ODF1 leads to structural abnormalities in the sperm tail and impaired motility, resulting in infertility. Researchers use ODF1 antibody to study spermatogenesis, sperm structure, and male fertility.
Clinically, ODF1 mutations or dysregulation have been associated with asthenozoospermia and male infertility. Studies in mice show that ODF1 knockout results in male sterility due to defective sperm tails. Because sperm tail defects are a common cause of infertility, ODF1 is a biomarker of reproductive health. NSJ Bioreagents provides ODF1 antibody for fertility and reproductive biology research.
Experimentally, ODF1 antibody is used in western blotting to detect the ~27 kDa protein, in immunofluorescence to visualize sperm tail localization, and in immunohistochemistry of testicular sections. Co-immunoprecipitation with ODF1 antibody reveals interactions with cytoskeletal partners critical for flagellar integrity. -
UniProt
- Q14990
抗原
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