GPD1 抗体 (N-Term)
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Quick Overview for GPD1 抗体 (N-Term) (ABIN7879605)
抗原
See all GPD1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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原理
- GPD1 Antibody / Glycerol-3-phosphate dehydrogenase 1
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纯化方法
- Immunogen affinity purified
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human GPD1 was used as the immunogen for the GPD1 antibody.
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亚型
- IgG
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anti-Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1) (AA 1-349) antibody
GPD1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3C10-1C4 unconjugated
anti-Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1) (AA 1-349) antibodyGPD1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1) (AA 28-57), (N-Term) antibodyGPD1 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB21027 unconjugated
anti-Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1) (AA 1-349) antibodyGPD1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1) (AA 35-65), (N-Term) antibodyGPD1 适用: 人, 小鼠 WB, FACS, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1) antibodyGPD1 适用: 人, 小鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1) (AA 1-349) antibodyGPD1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1) (AA 251-300) antibodyGPD1 适用: 人, 斑马鱼, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1) (AA 110-159) antibodyGPD1 适用: 人, 小鼠, 犬, 豚鼠, 马, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1) (N-Term) antibodyGPD1 适用: 人, 小鼠, 大鼠, 兔, 犬, 豚鼠, 马, Cow, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the GPD1 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the GPD1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- GPD1 (Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1))
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别名
- GPD1
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背景
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GPD1 antibody detects Glycerol-3-phosphate dehydrogenase 1, a cytosolic enzyme that catalyzes the reversible conversion of dihydroxyacetone phosphate to glycerol-3-phosphate, linking carbohydrate metabolism with lipid biosynthesis and energy regulation. GPD1 plays a crucial role in maintaining redox balance and metabolic flexibility. The GPD1 antibody is widely used in metabolic, biochemical, and endocrinological research to study lipid metabolism, redox cycling, and adipocyte differentiation.
GPD1 is encoded by the GPD1 gene located on human chromosome 12q13.12. The protein is approximately 349 amino acids long and localizes primarily in the cytoplasm. GPD1 functions in concert with its mitochondrial counterpart, GPD2, as part of the glycerophosphate shuttle that transfers reducing equivalents from NADH in the cytosol to the mitochondria for oxidative phosphorylation.
The GPD1 antibody detects a 37 kilodalton protein by western blot and shows cytosolic localization under immunofluorescence microscopy. GPD1 activity supports triglyceride synthesis in adipocytes and provides glycerol-3-phosphate as a substrate for lipid esterification. In muscle and liver, GPD1 contributes to energy metabolism by linking glycolysis to oxidative phosphorylation. Its regulation is sensitive to hormonal cues, including insulin and glucagon, and is modulated by redox state and nutrient availability.
Deficiency of GPD1 results in transient hypertriglyceridemia and hepatic steatosis due to impaired lipid processing. Genetic mutations have been associated with congenital lipodystrophy and metabolic syndrome. Increased GPD1 expression in adipose tissue correlates with obesity and insulin resistance, highlighting its importance in metabolic homeostasis.
Beyond energy metabolism, GPD1 influences cell signaling through regulation of NAD+/NADH balance and oxidative stress. It plays roles in thermogenesis, adipocyte differentiation, and lipid droplet biogenesis. NSJ Bioreagents provides a validated GPD1 antibody optimized for its applications, supporting studies into redox regulation, lipid metabolism, and energy homeostasis. -
UniProt
- P21695
抗原
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