Midline 2 抗体 (Middle Region)
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Quick Overview for Midline 2 抗体 (Middle Region) (ABIN7879527)
抗原
See all Midline 2 (MID2) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Middle Region
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原理
- MID2 Antibody / Midline 2
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纯化方法
- Immunogen affinity purified
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免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human MID2 was used as the immunogen for the MID2 antibody.
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亚型
- IgG
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anti-Midline 2 (MID2) (AA 506-735) antibody
MID2 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Midline 2 (MID2) (C-Term) antibodyVerified MID2 适用: 人 ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Midline 2 (MID2) (AA 251-350) antibodyMID2 适用: 人 ELISA, IF (cc), IF (p), IHC (p), IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated
anti-Midline 2 (MID2) (AA 690-717), (C-Term) antibodyMID2 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB33238 unconjugated
anti-Midline 2 (MID2) (AA 1-685) antibodyMID2 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Midline 2 (MID2) (C-Term) antibodyMID2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Midline 2 (MID2) (AA 210-259) antibodyMID2 适用: 人, 小鼠, 大鼠, 犬, 兔, 猴, 豚鼠, 马, Pig, Bat, Hamster WB 宿主: 兔 Polyclonal unconjugated
anti-Midline 2 (MID2) (AA 690-717), (C-Term) antibodyMID2 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the MID2 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the MID2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- Midline 2 (MID2)
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别名
- MID2
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背景
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MID2 antibody detects E3 ubiquitin-protein ligase MID2 (also known as Midline-2 or FXY2), a microtubule-associated E3 ligase involved in cytoskeletal organization, cell polarity, and developmental morphogenesis. Encoded by the MID2 gene on chromosome Xq22.3, this protein belongs to the tripartite motif (TRIM) family of ubiquitin ligases, featuring a RING finger domain, B-box zinc-binding motifs, a coiled-coil region, and a C-terminal COS domain that binds microtubules. MID2 functions in ubiquitination of cytoskeletal and signaling proteins, coordinating cell structure and trafficking processes crucial for morphogenesis and neurodevelopment.
In human cells, MID2 localizes along microtubules, at the centrosome, and in perinuclear regions. It associates with its close paralog MID1, with which it shares structural and functional similarities. Together, MID1 and MID2 regulate the stability of PP2A catalytic subunits and participate in tubulin network maintenance. MID2 expression is particularly high in brain, testis, and embryonic tissues, reflecting roles in neuronal migration and organ development. Mutations in MID2 have been associated with X-linked intellectual disability and developmental disorders affecting craniofacial and skeletal structures.
The MID2 antibody is widely used in cell biology and developmental research to study microtubule organization, ubiquitination, and cell morphology. Western blot analysis detects a ~78 kilodalton band corresponding to full-length MID2, while immunofluorescence reveals filamentous staining patterns overlapping with microtubules. MID2 ubiquitinates several substrates involved in cytoskeletal remodeling and signaling, including MID1-associated proteins and small GTPase regulators. Through these activities, it influences cell polarity, division, and migration. In neurons, MID2 contributes to axon guidance and growth cone dynamics.
Dysregulation of MID2 expression or mutation of its functional domains can lead to developmental abnormalities and neurological defects. Overexpression has been observed in certain cancers, where it may enhance cell motility and invasiveness through altered cytoskeletal signaling. The MID2 antibody provides a robust tool for investigating these pathways and identifying mechanisms of microtubule regulation in both normal and diseased states. NSJ Bioreagents supplies this antibody validated for its applications, supporting research into cytoskeletal dynamics, developmental disorders, and ubiquitin-dependent regulation. -
UniProt
- Q9UJV3
抗原
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