ARPIN 抗体 (Middle Region)
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Quick Overview for ARPIN 抗体 (Middle Region) (ABIN7879505)
抗原
See all ARPIN 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Middle Region
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原理
- ARPIN Antibody / Actin-related protein complex inhibitor
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纯化方法
- Immunogen affinity purified
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免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human ARPIN was used as the immunogen for the ARPIN antibody.
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亚型
- IgG
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anti-ARPIN (AA 1-226) antibody
适用: 人 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-ARPIN (N-Term) antibody适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-ARPIN (AA 58-107) antibody适用: 人, 小鼠, Cow, 犬, 马, Pig, 大鼠, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-ARPIN (N-Term) antibody适用: 人, 小鼠, Cow, 犬, 马, Pig, 大鼠, 豚鼠, 兔, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the ARPIN antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the ARPIN antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- ARPIN
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背景
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ARPIN antibody detects Actin-related protein complex inhibitor (Arpin), a negative regulator of actin filament branching that modulates cell motility and migration. The UniProt recommended name is Actin-related protein complex inhibitor (ARPIN). This cytoplasmic protein inhibits the Arp2/3 complex, controlling actin polymerization dynamics essential for lamellipodia formation, directional movement, and membrane protrusion regulation.
Functionally, ARPIN antibody identifies a 220-amino-acid protein that binds directly to the Arp2/3 complex through its C-terminal acidic motif, competing with activating factors such as WASP and WAVE. By inhibiting Arp2/3-dependent actin nucleation, ARPIN reduces protrusive force at the cell leading edge, promoting turning behavior and directional persistence. The protein is involved in establishing cell polarity, controlling migration speed, and maintaining cytoskeletal balance between extension and contraction.
The ARPIN gene (also known as C15orf38) is located on chromosome 15q24.1 and is expressed in multiple tissues, particularly in epithelial cells, fibroblasts, and neurons. Its activity is tightly regulated by upstream signaling through Rac1 and PI3K pathways that coordinate motility and cell shape remodeling.
Pathologically, altered ARPIN expression affects wound healing, cancer cell migration, and metastasis. Downregulation enhances cell motility and invasion by releasing inhibition of Arp2/3, while overexpression restricts lamellipodia formation and limits tumor spread. ARPIN thus acts as a molecular brake on cytoskeletal dynamics and metastatic progression. Research using ARPIN antibody supports studies in cytoskeletal biology, cancer metastasis, and motility regulation.
ARPIN antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect actin regulatory proteins. NSJ Bioreagents provides ARPIN antibody reagents optimized for studies in actin network modulation, cell migration, and intracellular signaling control.
Structurally, Actin-related protein complex inhibitor consists of an N-terminal acidic region, a coiled-coil central domain, and a conserved C-terminal WCA-like motif that mediates interaction with Arp2/3. This domain organization allows ARPIN to act as a direct inhibitor without requiring scaffolding cofactors. This antibody facilitates investigation of ARPIN's role in cytoskeletal remodeling and directional motility across diverse biological systems. -
UniProt
- Q7Z6K5
抗原
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