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Recombinant ANO1 抗体 (full length)

This 小鼠 单克隆 antibody specifically detects ANO1 in IHC (p). It exhibits reactivity toward 人.
产品编号 ABIN7877684
发货至: 中国
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Quick Overview for Recombinant ANO1 抗体 (full length) (ABIN7877684)

抗原

See all ANO1 抗体
ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

抗体类型

Recombinant Antibody

适用

  • 170
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  • 27
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  • 1
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宿主

  • 105
  • 70
小鼠

克隆类型

  • 105
  • 69
  • 1
单克隆

标记

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  • 14
  • 8
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  • 2
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  • 1
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  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
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This ANO1 antibody is un-conjugated

应用范围

  • 82
  • 61
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  • 50
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  • 21
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  • 16
  • 13
  • 13
  • 9
  • 3
  • 2
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  • 1
  • 1
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

RDG1-8750
  • 抗原表位

    • 27
    • 15
    • 8
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    • 3
    • 2
    • 2
    • 2
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    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
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    • 1
    full length

    原理

    Recombinant ANO1 Antibody / DOG1 / TMEM16A

    纯化方法

    Protein G affinity chromatography

    免疫原

    A recombinant human full length protein was used as the immunogen for this recombinant ANO1 antibody.

    亚型

    IgG2b, kappa
  • 应用备注

    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant ANO1 antibody to be titered up or down for optimal performance.

    1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.2 mg/mL

    缓冲液

    0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store the recombinant ANO1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
  • 抗原

    ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

    别名

    ANO1

    背景

    Expression of DOG1/ANO1 protein is elevated in the gastrointestinal stromal tumors (GIST's), c-kit signaling-driven mesenchymal tumors of the GI tract. It is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity for ANO1 has been reported in 97.8 percent of scorable GIST's, including all c-kit negative GIST's. Overexpression has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha mutants that fail to express c-kit antigen. The overall sensitivity of ANO1 and c-kit in GIST's is nearly identical: 94.4 % vs. 94.7 %.

    UniProt

    Q5XXA6
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