RAB27B 抗体 (C-Term)
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Quick Overview for RAB27B 抗体 (C-Term) (ABIN7877467)
抗原
See all RAB27B 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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原理
- RAB27B Antibody / Ras-related protein Rab-27B
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纯化方法
- Immunogen affinity purified
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human RAB27B was used as the immunogen for the RAB27B antibody.
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亚型
- IgG
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anti-RAB27B, Member RAS Oncogene Family (RAB27B) (AA 2-218) antibody
RAB27B 适用: 人 ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 2G11C2 unconjugated
anti-RAB27B, Member RAS Oncogene Family (RAB27B) (AA 1-218) antibodyRAB27B 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-RAB27B, Member RAS Oncogene Family (RAB27B) (C-Term) antibodyRAB27B 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 马, 兔, 猴, Pig, 绵羊, Hamster, 山羊, 驴, 小鸡, Cat, Avian WB, IHC (p), IF, IHC (fro) 宿主: 山羊 Polyclonal unconjugated
anti-RAB27B, Member RAS Oncogene Family (RAB27B) (AA 121-150) antibodyRAB27B 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB32417 unconjugated
anti-RAB27B, Member RAS Oncogene Family (RAB27B) (AA 186-215), (C-Term) antibodyRAB27B 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB24873 unconjugated
anti-RAB27B, Member RAS Oncogene Family (RAB27B) (AA 186-215) antibodyRAB27B 适用: 人 WB, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-RAB27B, Member RAS Oncogene Family (RAB27B) (AA 121-150) antibodyRAB27B 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-RAB27B, Member RAS Oncogene Family (RAB27B) antibodyRAB27B 适用: 人, 小鼠 WB, FACS 宿主: 小鼠 Monoclonal 4G7 unconjugated
anti-RAB27B, Member RAS Oncogene Family (RAB27B) (AA 1-218) antibodyRAB27B 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-RAB27B, Member RAS Oncogene Family (RAB27B) (C-Term) antibodyRAB27B 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the RAB27B antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the RAB27B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- RAB27B (RAB27B, Member RAS Oncogene Family (RAB27B))
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别名
- RAB27B
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背景
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RAB27B antibody detects Ras-related protein Rab-27B, a member of the Rab family of small GTPases that regulates vesicle docking, secretion, and exocytosis. RAB27B plays key roles in platelet dense granule release, melanosome transport, and hormone secretion. The RAB27B antibody is widely used in cell biology, endocrinology, and neurobiology to study regulated exocytosis and vesicle trafficking.
RAB27B is encoded by the RAB27B gene located on human chromosome 18q21.32. The protein is approximately 221 amino acids long and cycles between active GTP-bound and inactive GDP-bound states. RAB27B localizes to secretory vesicles and interacts with effector proteins such as SYTL2, MLPH, and Slac2 family members, facilitating vesicle tethering to the plasma membrane before exocytosis.
The RAB27B antibody detects a 25 kilodalton protein by western blot and shows vesicular punctate staining under confocal microscopy. RAB27B functions in a wide range of cell types, including platelets, endocrine cells, and neurons, where it regulates secretion of serotonin, insulin, and neurotransmitters. In melanocytes, RAB27B cooperates with RAB27A to control melanosome transport and pigment distribution.
Dysregulation of RAB27B has been linked to cancer metastasis, neurodegenerative disease, and immune dysfunction. Overexpression enhances invasive potential in breast and colorectal cancers by promoting secretion of exosomes that modify the tumor microenvironment. Mutations or reduced function lead to granule secretion defects and pigmentation abnormalities.
Because RAB27B regulates exocytosis and vesicle transport, it serves as a model for understanding intracellular trafficking mechanisms and communication between cells. NSJ Bioreagents provides a validated RAB27B antibody optimized for western blot, immunofluorescence, and vesicle transport assays, supporting studies in secretion, membrane dynamics, and cellular signaling. -
UniProt
- O00194
抗原
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