14-3-3 eta 抗体 (C-Term)
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Quick Overview for 14-3-3 eta 抗体 (C-Term) (ABIN7877305)
抗原
See all 14-3-3 eta (YWHAH) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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原理
- YWHAH Antibody / 14-3-3 eta
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纯化方法
- Immunogen affinity purified
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human YWHAH was used as the immunogen for the YWHAH antibody.
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亚型
- IgG
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anti-14-3-3 eta (YWHAH) (AA 4-246) antibody
YWHAH 适用: 人 WB, ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 eta (YWHAH) (AA 1-246) antibodyYWHAH 适用: 人 WB, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 eta (YWHAH) (AA 72-121) antibodyYWHAH 适用: 人, 小鼠, 大鼠, 猴, Cow, 兔, 犬, 斑马鱼, 小鸡, 非洲爪蟾, Bat, 豚鼠, 马, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 eta (YWHAH) antibodyYWHAH 适用: 人, 小鼠, 大鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 eta (YWHAH) (N-Term) antibodyYWHAH 适用: 人, 兔, 犬, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 eta (YWHAH) (N-Term) antibodyYWHAH 适用: 人, 小鼠, 大鼠, Cow, 兔, 小鸡, 非洲爪蟾 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 eta (YWHAH) (AA 71-170) antibodyYWHAH 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-14-3-3 eta (YWHAH) (AA 71-170) antibodyYWHAH 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 6A12 unconjugated
anti-14-3-3 eta (YWHAH) (Internal Region) antibodyYWHAH 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-14-3-3 eta (YWHAH) antibodyKO Validated YWHAH 适用: 人 WB 宿主: 小鼠 Monoclonal 25GB3955 unconjugated
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应用备注
- Optimal dilution of the YWHAH antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the YWHAH antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- 14-3-3 eta (YWHAH)
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别名
- YWHAH
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背景
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YWHAH antibody detects 14-3-3 protein eta, encoded by the YWHAH gene on chromosome 22q12.3. YWHAH antibody is widely applied in studies of signaling, apoptosis, and neurological disorders. YWHAH belongs to the 14-3-3 family of conserved regulatory proteins that bind phosphoserine-containing motifs on target proteins. Through these interactions, YWHAH regulates a wide range of cellular processes including cell cycle progression, apoptosis, signal transduction, and cytoskeletal organization.
Structurally, YWHAH is a ~28 kDa acidic protein that forms homo- or heterodimers with other 14-3-3 family members. Its structure consists of nine alpha-helices that create an amphipathic groove, enabling binding of phosphorylated motifs. These interactions influence localization, stability, and activity of client proteins. YWHAH is ubiquitously expressed, but highest in brain, reflecting its importance in neuronal signaling.
Functionally, YWHAH participates in multiple signaling cascades, including MAPK, PI3K-Akt, and apoptotic pathways. By binding pro-apoptotic proteins like BAD, YWHAH sequesters them in the cytoplasm to promote cell survival. It also modulates cytoskeletal dynamics by interacting with actin- and microtubule-regulatory proteins. YWHAH is involved in dopamine signaling by regulating proteins downstream of dopamine receptors. Researchers use YWHAH antibody to study signal transduction, apoptosis, and neurodegeneration.
Clinically, YWHAH is linked to neurological and psychiatric disorders. Genetic variants have been associated with schizophrenia, bipolar disorder, and Parkinson's disease. Its dysregulation is observed in neurodegenerative diseases such as Alzheimer's, where altered phosphorylation-dependent binding disrupts signaling. YWHAH is also studied in cancer, where its interactions influence cell proliferation and resistance to apoptosis. NSJ Bioreagents supplies YWHAH antibody to support signaling, neuroscience, and oncology research.
Experimentally, YWHAH antibody is used in western blotting to detect the ~28 kDa protein, in immunohistochemistry to analyze brain expression, and in immunofluorescence microscopy to visualize cytoplasmic localization. Co-immunoprecipitation with YWHAH antibody identifies client proteins across multiple pathways. -
UniProt
- Q04917
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途径
- Intracellular Steroid Hormone Receptor Signaling Pathway, C21-Steroid Hormone Metabolic Process, Myometrial Relaxation and Contraction
抗原
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