SYNM 抗体 (AA 93-1554)
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北京 101111
Quick Overview for SYNM 抗体 (AA 93-1554) (ABIN7877178)
抗原
See all SYNM 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 93-1554
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原理
- SYNM Antibody / Synemin
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human SYNM recombinant protein (Position: R93-D1554) was used as the immunogen for the SYNM antibody.
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亚型
- IgG
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anti-Synemin, Intermediate Filament Protein (SYNM) (AA 501-650) antibody
SYNM 适用: 小鼠, 大鼠 ELISA, WB, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Synemin, Intermediate Filament Protein (SYNM) (AA 1147-1417) antibodySYNM 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Synemin, Intermediate Filament Protein (SYNM) (AA 1466-1565) antibodySYNM 适用: 人 ELISA, WB 宿主: 小鼠 Monoclonal 4G5 unconjugated
anti-Synemin, Intermediate Filament Protein (SYNM) (AA 1350-1400) antibodySYNM 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the SYNM antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the SYNM antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- SYNM (Synemin, Intermediate Filament Protein (SYNM))
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别名
- SYNM
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背景
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The SYNM antibody targets Synemin, a large intermediate filament-associated protein encoded by the SYNM gene. Synemin belongs to the intermediate filament (IF) protein family and serves as a cytoskeletal crosslinker that connects desmin and vimentin filaments to cellular structures. Through these interactions, Synemin helps maintain cellular architecture, mechanical stability, and signaling dynamics. The SYNM antibody allows precise detection of Synemin to study cytoskeletal organization and mechanotransduction in muscle and non-muscle cells.
Synemin exists in multiple isoforms, including alpha and beta forms, which differ in size and tissue distribution. The alpha isoform is primarily expressed in striated muscle, while the beta isoform occurs in smooth muscle and non-muscle tissues such as astrocytes and hepatocytes. Both isoforms contain a conserved intermediate filamenti 1/2binding domain that mediates filament anchoring and a unique C-terminal tail that interacts with cytoskeletal and signaling proteins. The SYNM antibody detects these isoforms, enabling evaluation of Synemin expression across tissues and developmental stages.
Functionally, Synemin acts as a structural scaffold linking intermediate filaments to focal adhesions, costameres, and the plasma membrane. It binds to alpha-actinin, dystrophin, and protein kinase A regulatory subunits, integrating cytoskeletal mechanics with signal transduction. Loss or mutation of Synemin disrupts cell shape, elasticity, and contractility, emphasizing its role in maintaining structural integrity. Researchers employ the SYNM antibody to characterize these cytoskeletal connections and assess their impact on tissue strength and cellular signaling.
Synemin expression is prominent in cardiac and skeletal muscle, where it contributes to sarcomere alignment and force transmission. Dysregulation of Synemin may contribute to muscular dystrophies, cardiomyopathies, and other myopathic conditions. The SYNM antibody supports these investigations by providing a consistent and specific reagent for identifying Synemin in muscle biopsies, cultured myocytes, and tissue sections. Beyond muscle, Synemin has been implicated in glial and epithelial cell structure, suggesting roles in neurobiology and cancer progression.
Experimental applications of the SYNM antibody include western blotting, immunofluorescence, and immunohistochemistry. These assays reveal Synemin's filamentous distribution and dynamic rearrangement during cell migration or mechanical stress. NSJ Bioreagents supplies the SYNM antibody to facilitate ongoing research into cytoskeletal organization, signal transduction, and mechanobiology. Its reproducible specificity ensures reliable detection across experimental platforms, enabling detailed study of how Synemin contributes to cellular resilience, structure, and disease processes. -
UniProt
- O15061
抗原
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