NPRL2 抗体 (AA 90-380)
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Quick Overview for NPRL2 抗体 (AA 90-380) (ABIN7877107)
抗原
See all NPRL2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 90-380
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原理
- NPRL2 Antibody / Nitrogen permease regulator-like 2
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human NPRL2 recombinant protein (Position: D90-K380) was used as the immunogen for the NPRL2 antibody.
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亚型
- IgG
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anti-Nitrogen Permease Regulator-Like 2 (S. Cerevisiae) (NPRL2) (AA 1-380) antibody
NPRL2 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Nitrogen Permease Regulator-Like 2 (S. Cerevisiae) (NPRL2) (AA 1-220) antibodyNPRL2 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nitrogen Permease Regulator-Like 2 (S. Cerevisiae) (NPRL2) antibodyNPRL2 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nitrogen Permease Regulator-Like 2 (S. Cerevisiae) (NPRL2) (N-Term) antibodyNPRL2 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 兔, 斑马鱼, 非洲爪蟾, Hamster, 猴, Pig, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Nitrogen Permease Regulator-Like 2 (S. Cerevisiae) (NPRL2) (AA 1-220) antibodyNPRL2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Nitrogen Permease Regulator-Like 2 (S. Cerevisiae) (NPRL2) (N-Term) antibodyNPRL2 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 兔, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nitrogen Permease Regulator-Like 2 (S. Cerevisiae) (NPRL2) (Internal Region) antibodyVerified NPRL2 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Nitrogen Permease Regulator-Like 2 (S. Cerevisiae) (NPRL2) (C-Term) antibodyNPRL2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nitrogen Permease Regulator-Like 2 (S. Cerevisiae) (NPRL2) (AA 287-336) antibodyNPRL2 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 兔, Hamster, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Nitrogen Permease Regulator-Like 2 (S. Cerevisiae) (NPRL2) (N-Term) antibodyNPRL2 适用: 人, 小鼠, 大鼠, Cow, 犬, 斑马鱼, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the NPRL2 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the NPRL2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- NPRL2 (Nitrogen Permease Regulator-Like 2 (S. Cerevisiae) (NPRL2))
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别名
- NPRL2
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背景
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NPRL2 antibody detects Nitrogen permease regulator-like 2, a component of the GATOR1 complex that inhibits mTORC1 signaling in response to amino acid deprivation. NPRL2 functions as a tumor suppressor and metabolic checkpoint regulator, linking nutrient sensing to cell growth and autophagy. The NPRL2 antibody is widely used in metabolism, oncology, and signal transduction research to study mTOR regulation, nutrient signaling, and tumor suppression.
NPRL2 is encoded by the NPRL2 gene located on human chromosome 3p21.3, a region frequently deleted in several cancers. The protein is approximately 380 amino acids long and forms a complex with NPRL3 and DEPDC5 to constitute the GATOR1 inhibitory module. NPRL2 localizes to both cytoplasm and lysosomes, where it acts upstream of Rag GTPases to suppress mTORC1 activity under nutrient-limiting conditions.
The NPRL2 antibody detects a 45 kilodalton band by western blot and reveals punctate cytoplasmic staining consistent with lysosomal localization. NPRL2 is required for proper cellular adaptation to nutrient stress, promoting autophagy and metabolic reprogramming. Loss of NPRL2 results in constitutive mTORC1 activation, uncontrolled cell proliferation, and impaired stress response.
As a tumor suppressor, NPRL2 modulates DNA damage repair and enhances sensitivity to chemotherapeutic agents such as cisplatin. Reduced NPRL2 expression has been documented in lung, colorectal, and breast cancers, correlating with poor prognosis and therapy resistance. Restoring NPRL2 expression reactivates nutrient stress signaling and suppresses tumor growth through mTOR inhibition and apoptosis induction.
Through its dual role in nutrient sensing and cancer suppression, NPRL2 serves as a key regulator of cellular homeostasis. NSJ Bioreagents provides a validated NPRL2 antibody optimized for its applications, supporting research into mTOR signaling, autophagy regulation, and tumor suppression mechanisms. -
UniProt
- Q8WTW4
抗原
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