FAM178A 抗体 (AA 90-1157)
The 兔 多克隆 anti-FAM178A antibody is suitable to detect FAM178A in samples from 人, 小鼠 和 大鼠. It has been validated for ELISA, ICC, IF 和 WB.
产品编号 ABIN7877100
发货至:
中国
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Quick Overview for FAM178A 抗体 (AA 90-1157) (ABIN7877100)
抗原
FAM178A
(Family with Sequence Similarity 178, Member A (FAM178A))
适用
人, 小鼠, 大鼠
宿主
兔
克隆类型
多克隆
标记
This FAM178A antibody is un-conjugated
应用范围
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
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抗原表位
- AA 90-1157
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原理
- SLF2 Antibody / SMC5-SMC6 complex localization factor protein 2
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human SLF2 recombinant protein (Position: E90-N1157) was used as the immunogen for the SLF2 antibody.
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亚型
- IgG
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应用备注
- Optimal dilution of the SLF2 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the SLF2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- FAM178A (Family with Sequence Similarity 178, Member A (FAM178A))
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别名
- SLF2
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背景
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The SLF2 antibody targets SMC5-SMC6 complex localization factor 2, a nuclear protein encoded by the SLF2 gene that plays an essential role in DNA replication and repair. Also known as FAM178A, this protein helps recruit the SMC5/6 complex to chromatin following DNA damage, thereby promoting genome stability and replication fork recovery. SMC5-SMC6 complex localization factor 2 ensures proper DNA repair and cell cycle progression under genotoxic stress. The SLF2 antibody provides researchers with a precise tool to study DNA damage response pathways and chromosomal maintenance mechanisms.
SMC5-SMC6 complex localization factor 2 operates in coordination with its partner SLF1 to anchor the SMC5/6 complex at damaged replication forks. This localization facilitates homologous recombination repair and prevents collapse of stalled forks. The SLF2 antibody enables visualization of this critical repair factor in nuclear extracts and fixed cells, supporting detailed analysis of DNA repair kinetics and checkpoint activation. Its expression increases during replication stress and following exposure to DNA-damaging agents such as UV or ionizing radiation.
Disruption of SLF2 function compromises genomic integrity, leading to accumulation of DNA breaks, chromosomal abnormalities, and replication defects. Such genomic instability is a hallmark of cancer and aging. The SLF2 antibody aids in assessing how loss of SMC5-SMC6 recruitment affects DNA repair fidelity and cellular survival. In experimental models, depletion of SMC5-SMC6 complex localization factor 2 sensitizes cells to replication inhibitors, emphasizing its protective role during S phase.
Beyond replication stress response, SMC5-SMC6 complex localization factor 2 participates in maintaining telomere integrity and regulating chromatin compaction. The SLF2 antibody can be used to explore these broader nuclear processes, providing insight into how SMC5/6 and associated proteins orchestrate chromosomal architecture. Altered SLF2 expression has been implicated in tumorigenesis and therapy resistance, highlighting its relevance in cancer biology.
The SLF2 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, showing strong nuclear localization consistent with its DNA repair function. NSJ Bioreagents provides this antibody as a validated reagent with reliable specificity for chromatin and genome stability research. By enabling detailed examination of SMC5-SMC6 complex localization factor 2, the SLF2 antibody supports studies into DNA repair mechanisms, replication dynamics, and cellular responses to genomic stress. -
UniProt
- Q8IX21
抗原
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