Myotilin 抗体 (AA 9-494)
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Quick Overview for Myotilin 抗体 (AA 9-494) (ABIN7877090)
抗原
See all Myotilin (MYOT) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 9-494
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原理
- MYOT Antibody / Myotilin
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human MYOT recombinant protein (Position: H9-E494) was used as the immunogen for the MYOT antibody.
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亚型
- IgG
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anti-Myotilin (MYOT) (AA 259-314) antibody
MYOT 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Myotilin (MYOT) (C-Term) antibodyMYOT 适用: 人 IHC, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal RSO34 unconjugated
anti-Myotilin (MYOT) (AA 443-498) antibodyMYOT 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Myotilin (MYOT) (AA 209-221) antibodyVerified MYOT 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Myotilin (MYOT) (AA 127-155) antibodyMYOT 适用: 人 WB 宿主: 兔 Polyclonal RB36975 unconjugated
anti-Myotilin (MYOT) antibodyMYOT 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the MYOT antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the MYOT antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- Myotilin (MYOT)
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别名
- MYOT
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背景
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MYOT antibody detects Myotilin, a structural protein of the sarcomeric Z-disc that maintains muscle integrity and organization. Encoded by the MYOT gene on chromosome 5q31.2, this cytoskeletal protein binds alpha-actinin, filamin C, and other Z-disc components to stabilize actin filament cross-linking and support mechanical tension during muscle contraction. Myotilin plays a central role in maintaining sarcomere alignment, muscle elasticity, and myofibrillar assembly.
Structurally, Myotilin is a 57 kilodalton cytoplasmic protein containing two immunoglobulin-like domains at its C-terminus that mediate interactions with alpha-actinin and filamin. Its N-terminal serine-rich region supports actin binding and cross-linking, anchoring thin filaments at the Z-disc. Myotilin is expressed predominantly in skeletal and cardiac muscle, where it acts as a scaffold linking actin filaments to structural and signaling molecules that maintain contractile function.
The MYOT antibody is widely used in muscle biology, myopathy, and cytoskeletal research to study sarcomere structure, actin filament organization, and myofibrillogenesis. Western blot analysis identifies a 57 kilodalton band corresponding to Myotilin, while immunohistochemistry shows distinct Z-disc staining in muscle fibers. This antibody supports investigations into muscle development, maintenance, and degenerative conditions affecting the sarcomere.
Mutations in MYOT cause various myofibrillar myopathies, including limb-girdle muscular dystrophy type 1A and spheroid body myopathy. These mutations lead to Myotilin aggregation, sarcomeric disorganization, and progressive muscle weakness. Altered MYOT expression has also been linked to cardiac remodeling and hypertrophy. The MYOT antibody provides a critical tool for examining these pathogenic mechanisms and evaluating Myotilin's interactions with actin-binding and signaling proteins. NSJ Bioreagents validates this antibody for its applications, ensuring dependable results for muscle and cytoskeletal studies. -
UniProt
- Q9UBF9
抗原
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