PARP16 抗体 (AA 9-254)
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北京 101111
Quick Overview for PARP16 抗体 (AA 9-254) (ABIN7877069)
抗原
See all PARP16 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 9-254
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原理
- PARP16 Antibody / Poly ADP-ribose polymerase 16
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human PARP16 recombinant protein (Position: A9-Y254) was used as the immunogen for the PARP16 antibody.
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亚型
- IgG
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anti-Poly (ADP-Ribose) Polymerase Family, Member 16 (PARP16) (AA 215-244), (C-Term) antibody
PARP16 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB26311 unconjugated
anti-Poly (ADP-Ribose) Polymerase Family, Member 16 (PARP16) (N-Term) antibodyPARP16 适用: 人, 小鼠, 大鼠, 豚鼠, 马, Cow, 犬, Pig, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Poly (ADP-Ribose) Polymerase Family, Member 16 (PARP16) (AA 120-170) antibodyPARP16 适用: 人, 小鼠, 大鼠 WB, IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Poly (ADP-Ribose) Polymerase Family, Member 16 (PARP16) (AA 1-323) antibodyPARP16 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Poly (ADP-Ribose) Polymerase Family, Member 16 (PARP16) (AA 35-84) antibodyPARP16 适用: 人, 小鼠, 大鼠, 豚鼠, 马, Cow, Pig, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Poly (ADP-Ribose) Polymerase Family, Member 16 (PARP16) (AA 252-301) antibodyPARP16 适用: 人, 小鼠, 大鼠, 豚鼠, 马, 犬, 兔 WB 宿主: 兔 Polyclonal unconjugated
anti-Poly (ADP-Ribose) Polymerase Family, Member 16 (PARP16) (Middle Region) antibodyPARP16 适用: 人, 小鼠, 大鼠, 豚鼠, 马, Cow, 犬, Pig, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the PARP16 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the PARP16 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- PARP16 (Poly (ADP-Ribose) Polymerase Family, Member 16 (PARP16))
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别名
- PARP16
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背景
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PARP16 antibody detects Poly(ADP-ribose) polymerase 16, a membrane-associated mono-ADP-ribosyltransferase that regulates the unfolded protein response (UPR) and endoplasmic reticulum (ER) stress signaling. PARP16 belongs to the PARP family of enzymes involved in ADP-ribosylation, a post-translational modification that controls protein stability and signaling. The PARP16 antibody is commonly used in studies of ER stress, apoptosis, and ADP-ribosylation-dependent signaling pathways.
PARP16 is encoded by the PARP16 gene located on human chromosome 15q21.2. The protein is approximately 38 kilodaltons and uniquely localized to the ER membrane, where it functions as a tail-anchored enzyme with its catalytic domain facing the cytoplasm. Unlike canonical PARPs that catalyze poly(ADP-ribosylation), PARP16 primarily transfers single ADP-ribose moieties to substrate proteins (mono-ADP-ribosylation). This modification modulates the activity of key ER stress sensors, including PERK and IRE1-alpha, enhancing their signaling output during the UPR.
The PARP16 antibody typically detects a 38 kilodalton protein by western blot, and immunofluorescence confirms its ER localization. Functional studies demonstrate that PARP16 activity is induced upon ER stress caused by misfolded protein accumulation or chemical perturbation. Activated PARP16 promotes phosphorylation and activation of PERK and IRE1-alpha, leading to downstream transcriptional responses that restore proteostasis. Inhibition or depletion of PARP16 compromises the UPR, resulting in increased sensitivity to ER stress and enhanced apoptosis.
Beyond its role in ER stress regulation, PARP16 contributes to cancer cell survival and resistance to chemotherapeutic agents that induce proteotoxic stress. Overexpression has been reported in hepatocellular carcinoma, breast cancer, and melanoma, where it supports adaptive stress signaling. Conversely, loss of PARP16 sensitizes cells to stress-induced death, making it a potential therapeutic target. NSJ Bioreagents provides a validated PARP16 antibody optimized for immunofluorescence, immunohistochemistry and flow cytometry assays, enabling precise study of ADP-ribosylation mechanisms and ER homeostasis. -
UniProt
- Q8N5Y8
抗原
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