PRPF31 抗体 (AA 84-499)
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Quick Overview for PRPF31 抗体 (AA 84-499) (ABIN7876960)
抗原
See all PRPF31 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 84-499
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原理
- Zebrafish Prpf31 Antibody / Prp31 / Pre-mRNA processing factor 31
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纯化方法
- Antigen affinity chromatography
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免疫原
- E. coli-derived zebrafish Prpf31 recombinant protein (amino acids N84-K499) was used as the immunogen for the Zebrafish Prpf31 antibody.
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亚型
- Ig Fraction
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anti-Pre-mRNA Processing Factor 31 (PRPF31) (AA 400-499) antibody
PRPF31 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 8E1 unconjugated
anti-Pre-mRNA Processing Factor 31 (PRPF31) (AA 1-200) antibodyPRPF31 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Pre-mRNA Processing Factor 31 (PRPF31) antibodyPRPF31 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Pre-mRNA Processing Factor 31 (PRPF31) (AA 400-499) antibodyPRPF31 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Pre-mRNA Processing Factor 31 (PRPF31) (Internal Region) antibodyPRPF31 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Pre-mRNA Processing Factor 31 (PRPF31) (AA 449-499) antibodyPRPF31 适用: 人 WB, IHC (fp) 宿主: 兔 Polyclonal unconjugated
anti-Pre-mRNA Processing Factor 31 (PRPF31) (AA 1-499) antibodyPRPF31 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Pre-mRNA Processing Factor 31 (PRPF31) (Internal Region) antibodyVerified PRPF31 适用: 人 WB, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Pre-mRNA Processing Factor 31 (PRPF31) antibodyPRPF31 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
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应用备注
- Optimal dilution of the Zebrafish Prpf31 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the Zebrafish Prpf31 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- PRPF31 (Pre-mRNA Processing Factor 31 (PRPF31))
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别名
- Prpf31
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背景
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Prpf31 (Pre-mRNA processing factor 31) is a core component of the spliceosome, the complex responsible for removing introns from pre-messenger RNA transcripts. It is essential for the proper assembly and stability of the U4/U6-U5 tri-snRNP complex, a critical step in the formation of the mature spliceosome. By enabling efficient and accurate splicing of pre-mRNA, Prpf31 supports normal gene expression and cellular function.
In zebrafish, Prpf31 is the ortholog of the human PRPF31 gene. The zebrafish and human proteins are highly conserved, especially in functional domains that mediate interactions with other spliceosomal proteins. This conservation suggests that Prpf31 in zebrafish plays equivalent roles in pre-mRNA splicing and is suitable for modeling human splicing-related conditions.
Zebrafish Prpf31 may have multiple isoforms due to alternative splicing. These isoforms can contribute to functional diversity and may exhibit distinct spatial or temporal expression patterns during development. Isoform-specific roles are under ongoing investigation and may offer insights into tissue-specific splicing regulation.
Expression of Prpf31 in zebrafish is detected in tissues with high levels of transcriptional activity, such as the developing eye, brain, and somites. During early embryogenesis, its presence is crucial for the proper expression of genes required for organ development and cell differentiation.
Mutations in the human PRPF31 gene are associated with autosomal dominant retinitis pigmentosa, a degenerative eye disease. Zebrafish models have been used to study the pathogenesis of this condition and to explore therapeutic strategies, due to the gene’s conserved function and retinal expression.
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UniProt
- Q7SXM7
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途径
- Ribonucleoprotein Complex Subunit Organization
抗原
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