NGEF 抗体 (AA 82-627)
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北京 101111
Quick Overview for NGEF 抗体 (AA 82-627) (ABIN7876924)
抗原
See all NGEF 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 82-627
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原理
- NGEF Antibody / Ephexin 1
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human NGEF recombinant protein (Position: E82-Q627) was used as the immunogen for the NGEF antibody.
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亚型
- IgG
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anti-Neuronal Guanine Nucleotide Exchange Factor (NGEF) (AA 612-710) antibody
NGEF 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 6E7 unconjugated
anti-Neuronal Guanine Nucleotide Exchange Factor (NGEF) (AA 187-220), (N-Term) antibodyNGEF 适用: 人, 大鼠 WB 宿主: 兔 Polyclonal RB53236 unconjugated
anti-Neuronal Guanine Nucleotide Exchange Factor (NGEF) (AA 669-704), (C-Term) antibodyNGEF 适用: 人 WB 宿主: 兔 Polyclonal RB53235 unconjugated
anti-Neuronal Guanine Nucleotide Exchange Factor (NGEF) (AA 495-710) antibodyNGEF 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Neuronal Guanine Nucleotide Exchange Factor (NGEF) (AA 429-710) antibodyNGEF 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the NGEF antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the NGEF antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- NGEF (Neuronal Guanine Nucleotide Exchange Factor (NGEF))
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别名
- NGEF
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背景
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NGEF antibody detects Ephexin 1, encoded by the NGEF gene. Ephexin 1 is a guanine nucleotide exchange factor that regulates Rho family GTPases, controlling cytoskeletal dynamics, neuronal signaling, and axon guidance. NGEF antibody provides researchers with a specialized reagent for studying neuronal development, synaptic plasticity, and cell signaling.
Ephexin 1 is activated by ephrin receptor signaling, linking extracellular cues to intracellular Rho GTPase activity. Research using NGEF antibody has shown that it catalyzes GDP to GTP exchange on RhoA, Rac1, and Cdc42, thereby modulating actin cytoskeleton remodeling. These activities govern axon guidance during development, influencing neuronal wiring and brain connectivity.
Studies with NGEF antibody have revealed that Ephexin 1 is enriched in neurons, particularly in growth cones and synaptic regions. Its regulation by ephrin receptors ensures that axons respond appropriately to repulsive or attractive cues. This makes NGEF a critical player in neural circuit formation and brain development.
Dysregulation of Ephexin 1 has been linked to neurological disorders, including developmental delay, intellectual disability, and autism spectrum disorders. Research using NGEF antibody has shown that mutations or altered expression disrupt axon guidance and synaptic function, impairing neuronal communication. These findings highlight its clinical significance.
Beyond the nervous system, NGEF may play roles in cytoskeletal regulation in non-neuronal cells. Research using NGEF antibody has suggested functions in cell migration, immune cell trafficking, and tumor invasion. These roles broaden its biological relevance beyond neurodevelopment.
NGEF antibody is widely applied in immunohistochemistry, western blotting, and immunofluorescence. Immunohistochemistry highlights expression in brain tissue, western blotting quantifies protein levels across developmental stages, and immunofluorescence reveals subcellular localization at synapses. These applications make NGEF antibody indispensable for neurobiology research.
By supplying validated NGEF antibody reagents, NSJ Bioreagents supports studies into axon guidance, synaptic signaling, and disease. Detection of Ephexin 1 provides researchers with insight into how guanine nucleotide exchange factors regulate neuronal wiring and function. -
UniProt
- Q8N5V2
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途径
- Neurotrophin Signaling Pathway
抗原
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