INTS4 抗体 (AA 8-963)
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北京 101111
Quick Overview for INTS4 抗体 (AA 8-963) (ABIN7876850)
抗原
See all INTS4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 8-963
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原理
- INTS4 Antibody / Integrator complex subunit 4
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human INTS4 recombinant protein (Position: R8-R963) was used as the immunogen for the INTS4 antibody.
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亚型
- IgG
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anti-Integrator Complex Subunit 4 (INTS4) (AA 1-270) antibody
INTS4 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Integrator Complex Subunit 4 (INTS4) (AA 913-963) antibodyINTS4 适用: 人 IHC 宿主: 兔 Polyclonal unconjugated
anti-Integrator Complex Subunit 4 (INTS4) (AA 792-841) antibodyINTS4 适用: 人, 小鼠, 大鼠, 豚鼠, 兔, Cow, 犬, 马, Pig, Bat, Hamster, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Integrator Complex Subunit 4 (INTS4) (Middle Region) antibodyINTS4 适用: 人, 小鼠, 大鼠, Cow, 犬, 小鸡, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Integrator Complex Subunit 4 (INTS4) (AA 913-963) antibodyINTS4 适用: 人, 小鼠 WB, IP, IHC (fp) 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the INTS4 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the INTS4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- INTS4 (Integrator Complex Subunit 4 (INTS4))
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别名
- INTS4
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背景
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INTS4 antibody detects Integrator complex subunit 4, encoded by the INTS4 gene on chromosome 11p15.5. INTS4 antibody is widely used in studies of RNA processing, transcriptional regulation, and nuclear complexes. INTS4 is part of the Integrator complex, a multiprotein assembly that associates with RNA polymerase II and is essential for small nuclear RNA (snRNA) 3' end processing. By regulating snRNA maturation, INTS4 contributes to spliceosome function and overall gene expression control.
Structurally, INTS4 is a ~100 kDa protein that interacts with multiple Integrator subunits to stabilize complex assembly. It lacks obvious enzymatic domains but acts as a scaffold, organizing catalytic and RNA-binding subunits. Isoforms generated by alternative splicing may modify complex architecture or activity in specific tissues. Nuclear localization signals target INTS4 to sites of transcription and RNA processing.
Functionally, INTS4 coordinates snRNA 3' end formation, an essential step for spliceosome assembly and pre-mRNA splicing. It also contributes to regulation of enhancer RNA transcription and termination of certain protein-coding genes. Knockdown of INTS4 impairs Integrator stability, leading to widespread RNA processing defects. Researchers use INTS4 antibody to study transcriptional regulation, RNA biology, and spliceosome assembly.
Clinically, INTS4 mutations are associated with neurodevelopmental disorders, where defective RNA processing alters gene expression programs. Dysregulation of Integrator components has been implicated in cancer, particularly in transcription-driven tumors, where abnormal termination enhances oncogene expression. NSJ Bioreagents provides INTS4 antibody for studies of RNA processing, transcriptional regulation, and disease mechanisms.
Experimentally, INTS4 antibody is used in western blotting to detect the ~100 kDa protein, in immunoprecipitation to analyze Integrator complex composition, and in immunofluorescence microscopy to study nuclear localization. Chromatin immunoprecipitation with INTS4 antibody allows analysis of Integrator recruitment to active genes. -
UniProt
- Q96HW7
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途径
- SARS-CoV-2 Protein Interactome
抗原
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