CDCA8 抗体 (AA 72-280)
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北京 101111
Quick Overview for CDCA8 抗体 (AA 72-280) (ABIN7876633)
抗原
See all CDCA8 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 72-280
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原理
- CDCA8 Antibody / Cell division cycle-associated protein 8 / Borealin
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human CDCA8 recombinant protein (Position: D72-K280) was used as the immunogen for the CDCA8 antibody.
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亚型
- IgG
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anti-Cell Division Cycle Associated 8 (CDCA8) (AA 1-280) antibody
CDCA8 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Cell Division Cycle Associated 8 (CDCA8) (AA 1-280) antibodyCDCA8 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle Associated 8 (CDCA8) antibodyKD Validated CDCA8 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 24GB6600 unconjugated
anti-Cell Division Cycle Associated 8 (CDCA8) (AA 9-38), (N-Term) antibodyCDCA8 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB16561 unconjugated
anti-Cell Division Cycle Associated 8 (CDCA8) (AA 1-280) antibodyCDCA8 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle Associated 8 (CDCA8) (AA 1-280), (N-Term) antibodyCDCA8 适用: 人 WB, IF, IP 宿主: 小鼠 Monoclonal 1D11 unconjugated
anti-Cell Division Cycle Associated 8 (CDCA8) (AA 1-280) antibodyCDCA8 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle Associated 8 (CDCA8) (AA 1-124) antibodyCDCA8 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle Associated 8 (CDCA8) antibodyCDCA8 适用: 人 WB 宿主: 小鼠 Monoclonal 181CT41-2-3 unconjugated
anti-Cell Division Cycle Associated 8 (CDCA8) (Center) antibodyCDCA8 适用: 人 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the CDCA8 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the CDCA8 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- CDCA8 (Cell Division Cycle Associated 8 (CDCA8))
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别名
- CDCA8
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背景
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CDCA8 antibody recognizes Cell division cycle-associated protein 8, also known as Borealin, a core component of the chromosomal passenger complex (CPC) that governs chromosome segregation, spindle assembly, and cytokinesis. Encoded by the CDCA8 gene on chromosome 1p34.3, this protein interacts with Aurora B kinase, INCENP, and Survivin to form a functional complex that ensures accurate mitotic progression. Borealin is essential for proper kinetochore-microtubule attachment, spindle checkpoint activation, and correction of mitotic errors, thereby safeguarding genomic stability during cell division.
During mitosis, Borealin localizes to centromeres in metaphase and relocates to the spindle midzone during anaphase, coordinating the transition from chromosome alignment to cytokinesis. Loss of CDCA8 function results in chromosome missegregation, lagging chromatids, and multinucleation, phenotypes commonly associated with aneuploidy and cancer. Expression of CDCA8 peaks during the G2/M phase, reflecting its cell cycle-dependent regulation. Overexpression has been documented in a range of malignancies including breast, lung, and colorectal cancers, correlating with poor prognosis and high proliferative indices.
The CDCA8 antibody is widely used in cell cycle and cancer research to detect Borealin localization and expression dynamics. Immunofluorescence analysis with this antibody reveals distinct centromeric staining during metaphase and midbody localization in late mitosis. Western blotting identifies a 35 kilodalton band corresponding to the full-length protein. Because the chromosomal passenger complex regulates essential mitotic checkpoints, the CDCA8 antibody provides a critical tool for studying mechanisms of mitotic fidelity and tumorigenesis.
CDCA8 cooperates with Aurora B to phosphorylate substrates involved in spindle tension sensing and cytokinesis. Disruption of this interaction impairs CPC activity and leads to mitotic failure. Borealin's ability to bind DNA through its N-terminal domain and oligomerize via its coiled-coil region enables structural stability of the CPC. Inhibition or knockdown of CDCA8 sensitizes tumor cells to microtubule-targeting drugs, highlighting its potential as a therapeutic target. The antibody thus supports translational research exploring CPC inhibitors and mitotic checkpoint modulators in cancer therapy.
In developmental biology, CDCA8 is required for proper cell division during embryogenesis and organogenesis. The CDCA8 antibody facilitates identification of proliferating cells in rapidly dividing tissues and embryonic stem cell cultures. NSJ Bioreagents provides this antibody validated for its applications, ensuring consistent results in mitotic and oncogenic studies. -
UniProt
- Q53HL2
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途径
- Cell Division Cycle, M Phase
抗原
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