NSDHL 抗体 (AA 7-373)
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北京 101111
Quick Overview for NSDHL 抗体 (AA 7-373) (ABIN7876491)
抗原
See all NSDHL 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 7-373
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原理
- NSDHL Antibody / Sterol-4-alpha-carboxylate 3-dehydrogenase
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human NSDHL recombinant protein (Position: E7-K373) was used as the immunogen for the NSDHL antibody.
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亚型
- IgG
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anti-NAD(P) Dependent Steroid Dehydrogenase-Like (NSDHL) antibody
KD Validated NSDHL 适用: 人 WB, ICC, FACS 宿主: 兔 Monoclonal 24GB510 unconjugated Recombinant Antibody
anti-NAD(P) Dependent Steroid Dehydrogenase-Like (NSDHL) antibodyKD Validated NSDHL 适用: 人 WB, ICC, FACS 宿主: 兔 Monoclonal 24GB485 unconjugated Recombinant Antibody
anti-NAD(P) Dependent Steroid Dehydrogenase-Like (NSDHL) (AA 1-110) antibodyNSDHL 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 6E3 unconjugated
anti-NAD(P) Dependent Steroid Dehydrogenase-Like (NSDHL) (AA 1-240) antibodyKO Validated NSDHL 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-NAD(P) Dependent Steroid Dehydrogenase-Like (NSDHL) (AA 1-373) antibodyNSDHL 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-NAD(P) Dependent Steroid Dehydrogenase-Like (NSDHL) (AA 154-211) antibodyNSDHL 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-NAD(P) Dependent Steroid Dehydrogenase-Like (NSDHL) (AA 1-373) antibodyNSDHL 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-NAD(P) Dependent Steroid Dehydrogenase-Like (NSDHL) (AA 181-230) antibodyNSDHL 适用: 人, 大鼠, 小鼠, Cow, 犬, 豚鼠, 马, Pig, 兔, 斑马鱼, Bat, 小鸡, 猴, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the NSDHL antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the NSDHL antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- NSDHL (NAD(P) Dependent Steroid Dehydrogenase-Like (NSDHL))
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别名
- NSDHL
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背景
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NSDHL antibody detects Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating, a membrane-associated enzyme involved in cholesterol biosynthesis. The UniProt recommended name is Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (NSDHL). This enzyme catalyzes oxidative decarboxylation of sterol intermediates during the post-squalene steps of cholesterol production, playing a crucial role in lipid homeostasis and membrane integrity.
Functionally, NSDHL antibody identifies a 373-amino-acid enzyme localized to the endoplasmic reticulum membrane. NSDHL acts sequentially with other enzymes such as HSD17B7 and EBP to remove carboxyl groups from C4-methyl sterols, facilitating the conversion of lanosterol to cholesterol. Its catalytic activity contributes to maintaining sterol balance essential for cell signaling and membrane function.
The NSDHL gene is located on chromosome Xq28 and is highly expressed in liver, skin, and brain tissues. Its activity supports embryonic development, epidermal differentiation, and lipid raft formation in cell membranes. NSDHL is evolutionarily conserved and tightly regulated to ensure steady cholesterol output.
Pathologically, mutations in NSDHL cause CHILD syndrome (Congenital Hemidysplasia with Ichthyosiform erythroderma and Limb Defects), a rare X-linked disorder involving defective cholesterol metabolism and asymmetric skin and limb malformations. NSDHL dysfunction also disrupts sterol intermediates, leading to toxic accumulation of precursors. Research using NSDHL antibody supports studies in lipid metabolism, cholesterol biosynthesis, and genetic disorders of sterol processing.
NSDHL antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect sterol biosynthetic enzymes. NSJ Bioreagents provides NSDHL antibody reagents optimized for lipid biology, metabolism, and enzymology research.
Structurally, Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating contains a short-chain dehydrogenase/reductase catalytic domain and transmembrane segments that anchor it to the endoplasmic reticulum. This antibody enables study of NSDHL's role in sterol conversion and metabolic regulation. -
UniProt
- Q15738
抗原
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