NSMCE4A 抗体 (AA 69-380)
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北京 101111
Quick Overview for NSMCE4A 抗体 (AA 69-380) (ABIN7876445)
抗原
See all NSMCE4A 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 69-380
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原理
- NSMCE4A Antibody / Non-SMC element 4 homolog A
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human NSMCE4A recombinant protein (Position: D69-Q380) was used as the immunogen for the NSMCE4A antibody.
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亚型
- IgG
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anti-Non-SMC Element 4 Homolog A (NSMCE4A) (C-Term) antibody
NSMCE4A 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Non-SMC Element 4 Homolog A (NSMCE4A) (AA 29-57), (N-Term) antibodyNSMCE4A 适用: 人 WB 宿主: 兔 Polyclonal RB24938 unconjugated
anti-Non-SMC Element 4 Homolog A (NSMCE4A) (C-Term) antibodyNSMCE4A 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔, 斑马鱼 WB, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the NSMCE4A antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the NSMCE4A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- NSMCE4A (Non-SMC Element 4 Homolog A (NSMCE4A))
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别名
- NSMCE4A
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背景
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NSMCE4A antibody detects Non-structural maintenance of chromosomes element 4A, a core component of the SMC5/6 complex involved in chromosome maintenance, DNA repair, and replication fork stability. Encoded by the NSMCE4A gene on chromosome 10q25.3, this protein forms part of the essential SMC5/6 complex that ensures proper segregation of sister chromatids and preserves genome integrity during cell division and stress. NSMCE4A serves as a bridging subunit, connecting the SMC5 and SMC6 heterodimer to regulatory and enzymatic cofactors that control DNA repair and replication restart.
Structurally, NSMCE4A is a 34 kilodalton nuclear protein that interacts directly with SMC5 and SMC6 through its C-terminal coiled-coil domains, forming a stable hinge component of the complex. Its N-terminal region mediates associations with additional subunits such as NSMCE1 and NSMCE2, which confer ubiquitin ligase activity and DNA damage response signaling capacity. This modular organization enables NSMCE4A to coordinate complex assembly and spatial localization at damaged DNA sites.
The NSMCE4A antibody is widely used in molecular biology, DNA repair, and cell cycle research to study chromosomal maintenance, recombination, and replication dynamics. Western blot analysis identifies a 34 kilodalton band corresponding to NSMCE4A, while immunofluorescence shows punctate nuclear staining that increases following DNA damage. This antibody provides a powerful reagent for investigating the SMC5/6 complex and its role in genome stability.
Functionally, NSMCE4A is required for homologous recombination repair, resolution of DNA replication stress, and suppression of chromosomal rearrangements. Loss of NSMCE4A disrupts complex assembly, leading to DNA double-strand break accumulation and mitotic abnormalities. The SMC5/6 complex, including NSMCE4A, also regulates the maintenance of repetitive DNA sequences and prevents unscheduled recombination at telomeres and ribosomal DNA. Aberrant NSMCE4A expression or mutation has been implicated in genome instability disorders and cancer, where defective repair pathways promote genomic rearrangements. The NSMCE4A antibody enables sensitive detection and quantification of this repair factor in mechanistic studies of DNA integrity, replication stress, and chromosome segregation. NSJ Bioreagents validates this antibody for its applications, ensuring reproducible performance for DNA repair and chromosomal biology applications. -
UniProt
- Q9NXX6
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途径
- Positive Regulation of Response to DNA Damage Stimulus
抗原
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