OTUD6B 抗体 (AA 66-293)
Our Local Distributor
北京 101111
Quick Overview for OTUD6B 抗体 (AA 66-293) (ABIN7876335)
抗原
See all OTUD6B 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 66-293
-
原理
- OTUD6B Antibody / OTU domain-containing deubiquitinase 6B
-
纯化方法
- Immunogen affinity purified
-
免疫原
- E.coli-derived human OTUD6B recombinant protein (Position: E66-S293) was used as the immunogen for the OTUD6B antibody.
-
亚型
- IgG
-
-
anti-OTU Domain Containing 6B (OTUD6B) (AA 1-293) antibody
OTUD6B 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-OTU Domain Containing 6B (OTUD6B) (AA 1-293) antibodyOTUD6B 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3H4 unconjugated
anti-OTU Domain Containing 6B (OTUD6B) (AA 234-262), (C-Term) antibodyOTUD6B 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB27397 unconjugated
anti-OTU Domain Containing 6B (OTUD6B) (Middle Region) antibodyOTUD6B 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-OTU Domain Containing 6B (OTUD6B) (C-Term) antibodyOTUD6B 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔, 斑马鱼, Bat, 小鸡, Pig, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-OTU Domain Containing 6B (OTUD6B) antibodyOTUD6B 适用: 人, 小鼠 WB, FACS 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- Optimal dilution of the OTUD6B antibody should be determined by the researcher.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
-
缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- After reconstitution, the OTUD6B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
-
-
- OTUD6B (OTU Domain Containing 6B (OTUD6B))
-
别名
- OTUD6B
-
背景
-
OTUD6B antibody detects OTU domain-containing deubiquitinase 6B, a cysteine protease encoded by the OTUD6B gene located on chromosome 8q21.3. OTUD6B belongs to the ovarian tumor (OTU) domain-containing deubiquitinase family, which removes ubiquitin moieties from target proteins to regulate their stability, localization, and signaling activity. OTUD6B plays an important role in cellular protein homeostasis, DNA damage repair, and immune signaling by fine-tuning ubiquitin-mediated pathways. It is expressed in most tissues, with highest levels in brain, liver, and testis, reflecting its broad regulatory functions.
Structurally, OTUD6B contains an N-terminal OTU catalytic domain characterized by a conserved cysteine, histidine, and aspartate catalytic triad responsible for cleaving ubiquitin from substrates. It also includes regions for substrate recognition and potential regulatory phosphorylation sites. OTUD6B belongs to the OTU-type deubiquitinase family, which exhibits linkage-specific cleavage of polyubiquitin chains. Co-localization studies show OTUD6B primarily in the cytoplasm but also associated with the nucleus under stress conditions, suggesting roles in both cytosolic and nuclear signaling pathways.
Functionally, OTUD6B regulates protein degradation and signaling by removing ubiquitin from specific substrates targeted for proteasomal or lysosomal turnover. It modulates the stability of signaling mediators such as AKT, p53, and components of the mTOR pathway. OTUD6B also contributes to endoplasmic reticulum stress responses and DNA damage repair by reversing ubiquitination on repair factors. In immune cells, OTUD6B helps maintain homeostasis by controlling NF-?B activation and cytokine signaling. Known biochemical activities include cleavage of Lys48- and Lys63-linked ubiquitin chains, thereby influencing protein degradation and signaling transduction.
Deficiency or mutation of OTUD6B has been associated with intellectual developmental disorders, craniofacial abnormalities, and growth retardation, collectively known as OTUD6B-related neurodevelopmental syndrome. Dysregulation of OTUD6B expression has also been linked to tumor progression through effects on apoptosis and metabolic control. Pathway associations include ubiquitin-dependent protein catabolism, mTOR signaling, and cellular stress response. During development, OTUD6B supports cell growth and neuronal maturation by regulating key ubiquitinated targets involved in metabolism and transcription.
The OTUD6B antibody from NSJ Bioreagents is an excellent reagent for research on deubiquitination mechanisms, proteostasis, and signal transduction regulation. -
UniProt
- Q8N6M0
抗原
-
