SYT12 抗体 (AA 63-410)
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Quick Overview for SYT12 抗体 (AA 63-410) (ABIN7876240)
抗原
See all SYT12 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 63-410
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原理
- SYT12 Antibody / Synaptotagmin 12
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human Synaptotagmin-12/SYT12 recombinant protein (Position: Q63-R410) was used as the immunogen for the SYT12 antibody.
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亚型
- IgG
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anti-Synaptotagmin XII (SYT12) (N-Term) antibody
SYT12 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Synaptotagmin XII (SYT12) (AA 71-120) antibodySYT12 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Synaptotagmin XII (SYT12) (AA 47-150) antibodySYT12 适用: 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the SYT12 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the SYT12 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- SYT12 (Synaptotagmin XII (SYT12))
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别名
- SYT12
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背景
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SYT12 antibody detects Synaptotagmin 12, a member of the synaptotagmin family of membrane-trafficking proteins that regulate neurotransmitter release and synaptic vesicle exocytosis. Unlike calcium-sensing synaptotagmins, SYT12 lacks functional calcium-binding domains but modulates synaptic transmission by controlling vesicle docking and fusion probability. The SYT12 antibody is widely used in neurobiology and synaptic physiology to study vesicle release mechanisms, synaptic modulation, and neuronal signaling.
SYT12 is encoded by the SYT12 gene located on human chromosome 11q12.1. The protein is approximately 240 amino acids long and contains two C2-like domains typical of the synaptotagmin family. However, substitutions in key residues render these domains calcium-insensitive, distinguishing SYT12 as a regulator of synaptic machinery rather than a classical calcium sensor. SYT12 localizes predominantly to presynaptic terminals and associates with SNARE complex components that drive vesicle fusion.
An SYT12 antibody detects a 28 kilodalton band by western blot and reveals punctate staining at presynaptic sites under confocal microscopy. SYT12 interacts with SNAP25 and syntaxin, modulating the assembly of the SNARE complex and influencing vesicle priming efficiency. Experimental silencing of SYT12 in neurons decreases spontaneous and evoked neurotransmitter release, indicating its role in maintaining basal synaptic transmission.
Beyond synaptic function, SYT12 participates in hormone secretion and calcium-independent vesicle trafficking in endocrine cells. Mutations in SYT12 have been linked to neurological and psychiatric conditions, including epilepsy and movement disorders. In cancer research, SYT12 overexpression correlates with poor prognosis in lung and colorectal cancers, where it enhances tumor cell migration through altered vesicle dynamics.
SYT12 exemplifies the regulatory diversity of synaptotagmin family proteins, balancing vesicle fusion without directly sensing calcium. NSJ Bioreagents provides a validated SYT12 antibody optimized for its applications, supporting investigations into neurotransmission, vesicle priming, and secretory pathway control. -
UniProt
- Q8IV01
抗原
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