LRRC75A/FAM211A 抗体 (AA 52-292)
The 兔 多克隆 anti-LRRC75A/FAM211A antibody is suitable to detect LRRC75A/FAM211A in samples from 人. It has been validated for WB, ELISA 和 FACS.
产品编号 ABIN7875558
发货至:
中国
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Quick Overview for LRRC75A/FAM211A 抗体 (AA 52-292) (ABIN7875558)
抗原
LRRC75A/FAM211A (LRRC75A)
(Leucine Rich Repeat Containing 75A (LRRC75A))
适用
人
宿主
兔
克隆类型
多克隆
标记
This LRRC75A/FAM211A antibody is un-conjugated
应用范围
Western Blotting (WB), ELISA, Flow Cytometry (FACS)
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抗原表位
- AA 52-292
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原理
- LRRC75A Antibody / FAM211A
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纯化方法
- Antigen affinity purified
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免疫原
- An E.coli-derived human recombinant protein (amino acids H52-Q292) was used as the immunogen for the LRRC75A antibody.
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亚型
- IgG
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应用备注
- Optimal dilution of the LRRC75A antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the LRRC75A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- LRRC75A/FAM211A (LRRC75A) (Leucine Rich Repeat Containing 75A (LRRC75A))
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别名
- LRRC75A
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背景
- Long noncoding RNAs (lncRNAs) play multiple key roles during inflammatory processes. A novel lncRNA identified by the high-throughput sequencing analysis was found significantly down-regulated in Escherichia coli-introduced cell model of bovine mastitis. Given that this lncRNA consists of the antisense of leucine-rich repeat-containing protein 75A (LRRC75A), it was named LRRC75A antisense lncRNA1 (LRRC75A-AS1). The expression of LRRC75A-AS1 was down-regulated in bovine mammary epithelial cells and mammary tissues under inflammatory condition. Knockout (KO) of LRRC75A-AS1 by CRISPR-Cas9 system in bovine mammary alveolar cell-T (MAC-T) cell line could enhance expressions of tight junction (TJ) proteins Claudin-1, Occludin and ZO-1, reduce cell monolayer permeability, and inhibit Staphylococcus aureus adhesion and invasion. Meanwhile, it also down-regulated expressions of inflammatory factors and attenuated activation of NF-?B pathway. Similarly, knockdown of LRRC75A caused the changes as LRRC75A-AS1 KO did, while overexpression of LRRC75A enabled the opposite effects. TJ of epithelioid cells barriers the pathogenic microorganisms outside during inflammation, in which LRRC75A-AS1 can regulate the expression of TJ proteins through LRRC75A, affecting the development of inflammation.
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UniProt
- Q8NAA5
抗原
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