GALNT12 抗体 (AA 49-572)
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Quick Overview for GALNT12 抗体 (AA 49-572) (ABIN7875258)
抗原
See all GALNT12 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 49-572
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原理
- GALNT12 Antibody / Polypeptide N-acetylgalactosaminyltransferase 12
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human GALNT12 recombinant protein (Position: E49-Q572) was used as the immunogen for the GALNT12 antibody.
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亚型
- IgG
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anti-UDP-N-Acetyl-alpha-D-Galactosamine:polypeptide N-Acetylgalactosaminyltransferase 12 (GalNAc-T12) (GALNT12) (AA 1-272) antibody
GALNT12 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-UDP-N-Acetyl-alpha-D-Galactosamine:polypeptide N-Acetylgalactosaminyltransferase 12 (GalNAc-T12) (GALNT12) (AA 1-272) antibodyGALNT12 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-UDP-N-Acetyl-alpha-D-Galactosamine:polypeptide N-Acetylgalactosaminyltransferase 12 (GalNAc-T12) (GALNT12) antibodyGALNT12 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-UDP-N-Acetyl-alpha-D-Galactosamine:polypeptide N-Acetylgalactosaminyltransferase 12 (GalNAc-T12) (GALNT12) (AA 1-272) antibodyGALNT12 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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应用备注
- Optimal dilution of the GALNT12 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the GALNT12 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- GALNT12 (UDP-N-Acetyl-alpha-D-Galactosamine:polypeptide N-Acetylgalactosaminyltransferase 12 (GalNAc-T12) (GALNT12))
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别名
- GALNT12
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背景
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GALNT12 antibody detects Polypeptide N-acetylgalactosaminyltransferase 12, an enzyme involved in initiating mucin-type O-linked glycosylation of proteins. Encoded by the GALNT12 gene on chromosome 9q22.33, this Golgi-resident glycosyltransferase catalyzes the transfer of N-acetylgalactosamine (GalNAc) from UDP-GalNAc to serine or threonine residues on target proteins, forming the Tn antigen and enabling further elongation of O-glycan structures. GALNT12 plays a crucial role in regulating protein stability, secretion, and cell-cell communication through glycosylation control.
Structurally, GALNT12 contains a catalytic domain typical of the GalNAc-transferase family and a ricin-like lectin domain that mediates substrate recognition. It preferentially glycosylates substrates involved in epithelial integrity and mucin production. GALNT12 is expressed in gastrointestinal tissues and epithelial linings, where it influences mucin biosynthesis, cell adhesion, and signaling. Its activity affects the glycoprotein composition of the extracellular matrix and epithelial surfaces.
The GALNT12 antibody is widely used in glycobiology, cancer, and epithelial research to study O-glycosylation and mucin biosynthesis. Western blot analysis identifies a 60 kilodalton band corresponding to GALNT12, while immunofluorescence reveals perinuclear staining consistent with Golgi localization. This antibody is instrumental in evaluating changes in glycosylation during differentiation, inflammation, and tumorigenesis.
Mutations in GALNT12 are associated with colorectal cancer susceptibility, where altered O-glycosylation patterns affect cell adhesion and immune recognition. Dysregulated GALNT12 expression may lead to incomplete mucin glycosylation, contributing to tumor progression and epithelial dysfunction. The GALNT12 antibody supports research into glycosylation-dependent signaling, epithelial homeostasis, and cancer pathogenesis. NSJ Bioreagents offers this antibody validated for its applications, ensuring reliable performance in studies of glycoprotein biology and epithelial regulation. -
UniProt
- Q8IXK2
抗原
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