LMOD3 抗体 (AA 45-558)
The 兔 多克隆 anti-LMOD3 antibody (ABIN7875076) specifically detects LMOD3 in WB 和 ELISA.
The antibody is reactive with 人, 小鼠 和 大鼠 samples.
产品编号 ABIN7875076
发货至:
中国
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Quick Overview for LMOD3 抗体 (AA 45-558) (ABIN7875076)
抗原
LMOD3
(Leiomodin 3 (LMOD3))
适用
人, 小鼠, 大鼠
宿主
兔
克隆类型
多克隆
标记
This LMOD3 antibody is un-conjugated
应用范围
Western Blotting (WB), ELISA
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抗原表位
- AA 45-558
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原理
- LMOD3 Antibody / Leiomodin 3
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human LMOD3 recombinant protein (Position: D45-E558) was used as the immunogen for the LMOD3 antibody.
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亚型
- IgG
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anti-Leiomodin 3 (LMOD3) (AA 45-558) antibody
LMOD3 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Leiomodin 3 (LMOD3) (N-Term) antibodyLMOD3 适用: 人, Cow, 犬, 马 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the LMOD3 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the LMOD3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- LMOD3 (Leiomodin 3 (LMOD3))
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别名
- LMOD3
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背景
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LMOD3 antibody detects Leiomodin-3, a muscle-specific actin-binding protein that regulates thin filament assembly and sarcomere organization. The UniProt recommended name is Leiomodin-3 (LMOD3), a member of the leiomodin and tropomodulin family of actin filament regulators. LMOD3 is predominantly expressed in skeletal and cardiac muscle, where it acts as a potent nucleator and stabilizer of actin filaments critical for muscle contraction and structural integrity.
Functionally, LMOD3 antibody identifies a 579-amino-acid cytoskeletal protein that nucleates the formation of actin filaments by interacting with actin monomers and tropomyosin. Unlike tropomodulins, which cap the pointed ends of actin filaments to prevent elongation, LMOD3 promotes filament growth and organization, ensuring proper sarcomere length and alignment. Its C-terminal leucine-rich and WH2 domains bind actin directly, while an N-terminal region interacts with tropomyosin and other sarcomeric proteins. Through these interactions, LMOD3 maintains filament stability and alignment within the myofibrillar lattice.
The LMOD3 gene is located on chromosome 3p14.1 and encodes a protein essential for skeletal muscle development and maintenance. Mutations in LMOD3 cause nemaline myopathy type 10, a congenital disorder characterized by muscle weakness and the presence of nemaline bodies within myofibers. Defective LMOD3 leads to impaired thin filament elongation and disorganized sarcomeres, resulting in reduced muscle contractility and atrophy.
In normal muscle physiology, LMOD3 functions alongside leiomodin-1 and leiomodin-2, each serving tissue-specific roles in actin organization. LMOD3 localizes to the pointed ends of thin filaments near the M-line, complementing tropomodulin's capping activity at the same site. The balance between capping and elongation driven by LMOD3 and tropomodulin ensures optimal sarcomere structure and mechanical stability. In cardiac muscle, LMOD3 expression is induced during development and hypertrophic remodeling, indicating roles in both growth and adaptation.
LMOD3 antibody is commonly used to study muscle biology, sarcomere assembly, and actin cytoskeleton regulation. It is suitable for western blotting, immunofluorescence, and immunohistochemistry to detect LMOD3 expression in skeletal and cardiac tissues. Researchers utilize this antibody to investigate myopathies, cytoskeletal disorders, and mechanisms of muscle fiber regeneration. In vitro studies have shown that overexpression of LMOD3 enhances actin nucleation, while knockdown disrupts filament alignment and leads to myofibrillar disarray.
Structurally, LMOD3 contains a characteristic leiomodin family domain architecture with N-terminal tropomyosin-binding regions, central coiled-coil motifs, and C-terminal WH2 domains for actin nucleation. It also undergoes phosphorylation-dependent regulation that modulates its actin-binding affinity. NSJ Bioreagents provides LMOD3 antibody reagents validated for use in muscle research, cytoskeletal dynamics, and structural biology. -
UniProt
- Q0VAK6
抗原
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