SEC63 抗体 (AA 37-760)
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Quick Overview for SEC63 抗体 (AA 37-760) (ABIN7874418)
抗原
See all SEC63 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 37-760
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原理
- SEC63 Antibody / DNAJC23
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human SEC63 recombinant protein (Position: R37-D760) was used as the immunogen for the SEC63 antibody.
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亚型
- IgG
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anti-SEC63 Homolog (SEC63) (AA 631-728) antibody
SEC63 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1A8 unconjugated
anti-SEC63 Homolog (SEC63) (AA 1-760) antibodySEC63 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-SEC63 Homolog (SEC63) (C-Term) antibodySEC63 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-SEC63 Homolog (SEC63) (AA 139-168), (N-Term) antibodySEC63 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB32397 unconjugated
anti-SEC63 Homolog (SEC63) antibodySEC63 适用: 人, 小鼠, 犬 WB 宿主: 兔 Polyclonal unconjugated
anti-SEC63 Homolog (SEC63) (AA 139-168) antibodySEC63 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-SEC63 Homolog (SEC63) (AA 651-700) antibodySEC63 适用: 人, 小鼠, 大鼠, 犬, 豚鼠, 兔, 斑马鱼, Cow, 马, Bat, 猴, 小鸡, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-SEC63 Homolog (SEC63) (AA 35-84) antibodySEC63 适用: 人, 小鼠, 大鼠, 犬, 豚鼠, 兔, 斑马鱼, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-SEC63 Homolog (SEC63) (AA 700-750) antibodySEC63 适用: 人, 小鼠 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-SEC63 Homolog (SEC63) (N-Term) antibodySEC63 适用: 人, 小鼠, 大鼠, 豚鼠, 兔, 斑马鱼, Cow, 马 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the SEC63 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the SEC63 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- SEC63 (SEC63 Homolog (SEC63))
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别名
- SEC63
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背景
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SEC63 antibody detects Sec63 homolog, a component of the endoplasmic reticulum (ER) translocon complex encoded by the SEC63 gene on chromosome 6q21. SEC63 is an integral membrane protein essential for protein translocation into the ER and is conserved from yeast to humans. It functions as part of the SEC61/SEC62/SEC63 complex, which mediates post-translational import of secretory and membrane proteins into the ER lumen. SEC63 interacts directly with BiP (HSPA5), the ER-resident chaperone, to facilitate ATP-driven polypeptide movement across the translocon pore. The presence of SEC63 ensures proper folding and maturation of newly synthesized proteins, thereby maintaining proteostasis and ER homeostasis.
Structurally, SEC63 is a multi-pass membrane protein with a luminal J-domain that binds BiP. This J-domain stimulates BiP's ATPase activity, coordinating polypeptide translocation and preventing premature folding. SEC63 also contains hydrophobic transmembrane segments that anchor it within the ER and permit association with SEC61 alpha, beta, and gamma subunits. Loss-of-function mutations in SEC63 disrupt ER import, leading to protein misfolding and accumulation, hallmarks of ER stress and unfolded protein response activation.
Clinically, SEC63 is associated with polycystic liver disease (PCLD). Mutations in SEC63 impair secretory protein maturation, particularly affecting hepatocyte glycoproteins, leading to cyst formation and progressive liver dysfunction. Animal models with SEC63 deficiency replicate these phenotypes, confirming its pathogenic role. Moreover, SEC63 has been implicated in cancer biology: dysregulated expression alters secretory pathways and contributes to tumor growth, metastasis, and altered stress signaling. SEC63 antibody is therefore a valuable research tool in hepatology, ER biology, and cancer studies.
Experimentally, SEC63 antibody is used for western blotting, immunoprecipitation, immunofluorescence, and immunohistochemistry. In cultured cells, immunostaining shows SEC63 localization in the perinuclear ER network, while biochemical assays reveal its incorporation into SEC61 complexes. Researchers employ SEC63 antibody to investigate ER import fidelity, stress responses, and disease-related mutations. In clinical research, SEC63 detection provides insights into genetic causes of liver cysts and potential therapeutic approaches targeting protein folding pathways. NSJ Bioreagents provides SEC63 antibody to ensure consistent, validated detection for these applications. -
UniProt
- Q9UGP8
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途径
- ER-Nucleus Signaling
抗原
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