PIK3R4 抗体 (AA 36-1339)
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Quick Overview for PIK3R4 抗体 (AA 36-1339) (ABIN7874291)
抗原
See all PIK3R4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 36-1339
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原理
- PIK3R4 Antibody / Phosphoinositide 3-kinase regulatory subunit 4
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纯化方法
- Antigen affinity chromatography
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免疫原
- An E.coli-derived human recombinant protein (amino acids R36-Q1339) was used as the immunogen for the PIK3R4 antibody.
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亚型
- IgG
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anti-Phosphoinositide 3-kinase Regulatory Subunit 4 (PIK3R4) (AA 1259-1358) antibody
PIK3R4 适用: 人 ELISA, WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 1B5 unconjugated
anti-Phosphoinositide 3-kinase Regulatory Subunit 4 (PIK3R4) (AA 1259-1358) antibodyPIK3R4 适用: 人 ELISA, WB, RNAi 宿主: 小鼠 Monoclonal 1D4 unconjugated
anti-Phosphoinositide 3-kinase Regulatory Subunit 4 (PIK3R4) (AA 772-906) antibodyPIK3R4 适用: 人 ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 5H3A11 unconjugated
anti-Phosphoinositide 3-kinase Regulatory Subunit 4 (PIK3R4) antibodyPIK3R4 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Phosphoinositide 3-kinase Regulatory Subunit 4 (PIK3R4) (AA 1300-1358), (Regulatory Subunit) antibodyPIK3R4 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Phosphoinositide 3-kinase Regulatory Subunit 4 (PIK3R4) (AA 772-906) antibodyPIK3R4 适用: 人 ELISA 宿主: 小鼠 Monoclonal 2C11E8 unconjugated
anti-Phosphoinositide 3-kinase Regulatory Subunit 4 (PIK3R4) antibodyKD Validated PIK3R4 适用: 人 WB, FACS 宿主: 兔 Monoclonal 25GB325 unconjugated Recombinant Antibody
anti-Phosphoinositide 3-kinase Regulatory Subunit 4 (PIK3R4) (AA 1259-1358) antibodyPIK3R4 适用: 人 ELISA, WB 宿主: 小鼠 Monoclonal 2E7 unconjugated
anti-Phosphoinositide 3-kinase Regulatory Subunit 4 (PIK3R4) (AA 1-330) antibodyPIK3R4 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Phosphoinositide 3-kinase Regulatory Subunit 4 (PIK3R4) (AA 53-83), (N-Term) antibodyPIK3R4 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB1721 unconjugated
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应用备注
- Optimal dilution of the PIK3R4 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the PIK3R4 Antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- PIK3R4 (Phosphoinositide 3-kinase Regulatory Subunit 4 (PIK3R4))
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别名
- PIK3R4
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背景
- Phosphoinositide 3-kinase regulatory subunit 4, also known as PI3-kinase regulatory subunit 4 or PI3-kinase p150 subunit or phosphoinositide 3-kinase adaptor protein, or VPS15 is an enzyme that in humans is encoded by the PIK3R4 gene. The serine/threonine PI3 kinase regulatory subunit 4 (PIK3R4, Vps15) is the mammalian homologue of the yeast vacuolar protein sorting 15. PIK3R4 regulates the kinase activity of PI3K class III and anchors the kinase to cellular membranes through myristoylation. Recruitment of PI3K class III to the site of early endosome fusion and docking is directly mediated by PIK3R4 binding to the small GTPase Rab5 through its HEAT and WD-40 domains. The PIK3R4/PI3K class III plays a role in late endosome function through PIK3R4 binding to the Rab7 GTPase. In addition to its role in trafficking, the PIK3R4/PI3K class III complex interacts with beclin-1 to play a role during several stages of autophagy. Autophagosome formation is stimulated when Atg14 complexes with PIK3R4, PI3K class III, and beclin-1. The UVRAG protein competes with Atg14 for beclin-1 binding, forming a mutually exclusive complex with PIK3R4, PI3K class III, and beclin-1 that regulates autophagosome maturation. Autophagosome maturation is impaired in the presence of the beclin-1-binding protein Rubicon. Co-expression of PIK3R4 is required for PI3K class III activation and regulation by both beclin-1/UVRAG and by nutrients. Overexpression of PIK3R4 protein has been associated with decreased survival in patients with ovarian tumors, while mutations of the corresponding PIK3R4 gene are associated with metastatic melanoma, suggesting that PIK3R4 functions in cancer.
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UniProt
- Q99570
抗原
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