ITGA4 抗体 (AA 35-269)
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Quick Overview for ITGA4 抗体 (AA 35-269) (ABIN7874159)
抗原
See all ITGA4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 35-269
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原理
- ITGA4 Antibody / Integrin Alpha 4
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human Integrin Alpha 4/ITGA4 recombinant protein (Position: N35-V269) was used as the immunogen for the ITGA4 antibody.
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亚型
- IgG
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anti-Integrin alpha 4 (ITGA4) (Internal Region) antibody
ITGA4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha 4 (ITGA4) antibodyITGA4 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 5C4 unconjugated
anti-Integrin alpha 4 (ITGA4) (AA 551-650) antibodyITGA4 适用: 人, 小鼠, 兔 FACS, WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha 4 (ITGA4) (AA 833-977) antibodyITGA4 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 7E4C5 unconjugated
anti-Integrin alpha 4 (ITGA4) (AA 376-558) antibodyITGA4 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha 4 (ITGA4) (C-Term) antibodyITGA4 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha 4 (ITGA4) antibodyITGA4 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal unconjugated
anti-Integrin alpha 4 (ITGA4) antibodyKO Validated ITGA4 适用: 人 FACS, WB 宿主: 兔 Monoclonal 25GB2175 unconjugated Recombinant Antibody
anti-Integrin alpha 4 (ITGA4) (AA 999-1028), (C-Term) antibodyITGA4 适用: 人, 大鼠 FACS, WB, IHC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha 4 (ITGA4) antibodyITGA4 适用: 人 FACS, WB, EIA 宿主: 小鼠 Monoclonal 5C4 unconjugated
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应用备注
- Optimal dilution of the ITGA4 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the ITGA4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- ITGA4 (Integrin alpha 4 (ITGA4))
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别名
- ITGA4
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背景
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Integrin alpha 4 antibody detects Integrin alpha-4 (encoded by ITGA4), a cell surface adhesion receptor that mediates interactions between cells and the extracellular matrix, primarily through binding to vascular cell adhesion molecule 1 (VCAM1) and fibronectin. Encoded by the ITGA4 gene on chromosome 2q31.3, this protein pairs with the beta-1 (ITGB1) or beta-7 (ITGB7) subunit to form functional integrin heterodimers such as alpha4beta1 (VLA-4) and alpha4beta7. These complexes regulate leukocyte adhesion, migration, and immune cell trafficking across vascular and epithelial barriers.
Integrin alpha-4 is a transmembrane glycoprotein composed of a large extracellular domain, a single transmembrane helix, and a short cytoplasmic tail that links to cytoskeletal and signaling molecules. It facilitates rolling and firm adhesion of lymphocytes and monocytes during inflammatory responses, guiding immune cells to sites of tissue injury or infection. In the nervous system, it contributes to axonal guidance and glial cell adhesion.
The ITGA4 antibody is widely used in immunology, oncology, and vascular biology research to study adhesion, immune cell migration, and integrin-mediated signaling. Flow cytometry and western blot analyses identify Integrin alpha-4 as a 150 kilodalton band, while immunohistochemistry reveals membrane staining on leukocytes, endothelium, and fibroblasts. This antibody provides a means to examine integrin expression dynamics and cell adhesion regulation under physiological and pathological conditions.
Integrin alpha-4 is clinically relevant as a therapeutic target in multiple sclerosis and Crohn's disease, where monoclonal antibodies blocking alpha4-integrin (e.g., natalizumab) reduce leukocyte infiltration and inflammation. Aberrant expression also contributes to cancer metastasis and fibrotic diseases by enhancing cell adhesion and migration. The ITGA4 antibody supports investigations into these mechanisms by detecting endogenous protein in tissues and cell lines. NSJ Bioreagents offers this antibody validated for its applications, ensuring specificity and sensitivity for integrin research. -
UniProt
- P13612
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途径
- Integrin Complex
抗原
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