Hephaestin 抗体 (AA 348-1157)
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Quick Overview for Hephaestin 抗体 (AA 348-1157) (ABIN7874122)
抗原
See all Hephaestin (HEPH) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 348-1157
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原理
- Hephaestin Antibody / HEPH
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纯化方法
- Antigen affinity purified
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免疫原
- A human recombinant partial protein (amino acids E348-Q1157) was used as the immunogen for the Hephaestin antibody.
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亚型
- IgG
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anti-Hephaestin (HEPH) (AA 315-424) antibody
HEPH 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2D3 unconjugated
anti-Hephaestin (HEPH) (AA 21-120) antibodyHEPH 适用: 人, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated
anti-Hephaestin (HEPH) (AA 300-580) antibodyHEPH 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Hephaestin (HEPH) (AA 315-424) antibodyHEPH 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
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应用备注
- Optimal dilution of the Hephaestin antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the Hephaestin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- Hephaestin (HEPH)
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别名
- Hephaestin
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背景
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Hephaestin (HEPH) is a multicopper ferroxidase that plays a critical role in systemic iron homeostasis. It is primarily expressed in intestinal enterocytes, where it facilitates the export of dietary iron into the bloodstream. Hephaestin functions by oxidizing ferrous iron (Fe2+) to ferric iron (Fe3+), a form that can be bound by transferrin for transport throughout the body. Researchers often use a Hephaestin antibody to study iron metabolism, absorption, and related disorders.
The activity of Hephaestin is closely linked to ferroportin, the only known iron exporter in mammals. By converting Fe2+ to Fe3+, Hephaestin stabilizes iron export and prevents the accumulation of reactive iron species that could cause oxidative stress. Employing a Hephaestin antibody allows scientists to investigate how this protein cooperates with ferroportin and other regulators of iron homeostasis, such as hepcidin and ceruloplasmin.
Mutations in the HEPH gene are associated with iron transport defects and conditions such as anemia and iron overload. Animal models with disrupted Hephaestin function exhibit impaired dietary iron absorption, underscoring its physiological importance. Beyond its intestinal role, Hephaestin has also been detected in other tissues, including the brain and retina, where it may influence neuronal iron balance and oxidative stress responses. Using a Hephaestin antibody supports research into both systemic and tissue-specific aspects of iron regulation.
NSJ Bioreagents provides a high-quality Hephaestin antibody validated for applications such as western blot, immunohistochemistry, and immunofluorescence. Choosing a Hephaestin antibody from NSJ Bioreagents ensures reliable performance and reproducibility in studies of iron transport, metabolism, and disease. -
UniProt
- Q9BQS7
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途径
- Transition Metal Ion Homeostasis
抗原
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