MST1 抗体 (AA 324-452)
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Quick Overview for MST1 抗体 (AA 324-452) (ABIN7873971)
抗原
See all MST1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 324-452
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原理
- MST1 Antibody / Mammalian STE20-like protein kinase 1 / STK4
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human MST1/STK4 recombinant protein (Position: E324-D452) was used as the immunogen for the MST1 antibody.
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亚型
- IgG
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anti-Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like) (MST1) (AA 385-487) antibody
MST1 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like) (MST1) (AA 200-500) antibodyMST1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like) (MST1) (AA 385-415), (C-Term) antibodyMST1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB1193 unconjugated
anti-Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like) (MST1) (AA 454-483) antibodyMST1 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like) (MST1) (AA 489-716) antibodyMST1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like) (MST1) (C-Term) antibodyMST1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like) (MST1) (AA 454-483), (C-Term) antibodyMST1 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB31051 unconjugated
anti-Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like) (MST1) (AA 1-711) antibodyMST1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like) (MST1) (AA 363-465) antibodyMST1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like) (MST1) (AA 540-589) antibodyMST1 适用: 人, 犬, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the MST1 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the MST1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- MST1 (Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like) (MST1))
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别名
- MST1
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背景
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MST1 antibody detects Mammalian STE20-like protein kinase 1, a serine/threonine kinase that functions as a central regulator of apoptosis, stress signaling, and organ size control. The UniProt recommended name is Serine/threonine-protein kinase 4 (STK4), commonly referred to as MST1. This kinase belongs to the Hippo signaling pathway, a conserved network that restricts cell proliferation and promotes programmed cell death to maintain tissue homeostasis.
Functionally, MST1 antibody identifies a 487-amino-acid cytoplasmic kinase that becomes activated in response to oxidative stress, DNA damage, or cell detachment. Upon activation, MST1 autophosphorylates and phosphorylates downstream kinases such as LATS1/2, initiating a signaling cascade that culminates in the phosphorylation and inactivation of the transcriptional co-activators YAP and TAZ. This process suppresses genes promoting cell proliferation and survival. Additionally, MST1 can translocate to the nucleus, where it phosphorylates histone H2B and FOXO transcription factors to promote apoptosis and DNA repair.
The MST1 gene is located on chromosome 20q13.12 and encodes a key component of the mammalian Hippo pathway. MST1 activity is tightly regulated through interactions with adaptor proteins including SAV1 and MOB1, which enhance its kinase activity and substrate recognition. The pathway acts as a molecular brake on organ growth and tumorigenesis by balancing proliferation and cell death. Loss or mutation of MST1 disrupts Hippo signaling, leading to uncontrolled tissue growth and increased cancer risk.
In addition to its role in growth control, MST1 regulates immune function by modulating T-cell migration and survival. It promotes lymphocyte trafficking by controlling actin cytoskeleton organization and integrin-mediated adhesion. MST1 deficiency in humans results in primary immunodeficiency characterized by recurrent infections and defective T-cell activation. In neurons, MST1 contributes to axonal degeneration under oxidative stress, linking it to neurodegenerative disease mechanisms.
MST1 antibody is widely used in research on apoptosis, signal transduction, and cancer biology. It is suitable for immunoblotting, immunoprecipitation, and fluorescence microscopy to monitor MST1 activation, phosphorylation, and subcellular localization. In cancer research, MST1 serves as a tumor suppressor marker, while in neuroscience, it is investigated for its role in neuronal apoptosis. The antibody supports studies on kinase cascades that control organ size, cytoskeletal remodeling, and oxidative stress response.
Structurally, MST1 contains an N-terminal kinase domain and a C-terminal SARAH domain responsible for dimerization and interaction with Hippo pathway components. Post-translational modifications including autophosphorylation, caspase-mediated cleavage, and ubiquitination regulate its activity and localization. NSJ Bioreagents provides MST1 antibody reagents validated for use in apoptosis, Hippo signaling, and kinase pathway research. -
UniProt
- Q13043
抗原
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