Pumilio 3 抗体 (AA 318-631)
The 兔 多克隆 anti-Pumilio 3 antibody (ABIN7873900) specifically detects Pumilio 3 in ELISA, WB 和 FACS.
The antibody is reactive with 人 samples.
产品编号 ABIN7873900
发货至:
中国
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Quick Overview for Pumilio 3 抗体 (AA 318-631) (ABIN7873900)
抗原
Pumilio 3 (PUM3)
适用
人
宿主
兔
克隆类型
多克隆
标记
This Pumilio 3 antibody is un-conjugated
应用范围
ELISA, Western Blotting (WB), Flow Cytometry (FACS)
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抗原表位
- AA 318-631
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原理
- PUM3 Antibody / Pumilio 3
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human PUF-A/KIAA0020/PUM3 recombinant protein (Position: Q318-K631) was used as the immunogen for the PUM3 antibody.
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亚型
- IgG
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anti-Pumilio 3 (PUM3) antibody
PUM3 适用: 人 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Pumilio 3 (PUM3) antibodyPUM3 适用: 人 ELISA, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the PUM3 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the PUM3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- Pumilio 3 (PUM3)
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别名
- PUM3
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背景
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PUM3 antibody detects Pumilio homolog 3 (also known as Pumilio domain-containing protein 3), an RNA-binding protein that participates in post-transcriptional gene regulation and ribosome biogenesis. Encoded by the PUM3 gene on chromosome 9q34.11, this protein is a member of the PUF (Pumilio and FBF) family characterized by their RNA recognition repeats that bind specific sequences in target transcripts. Unlike classical cytoplasmic Pumilio proteins (PUM1 and PUM2), PUM3 is localized to the nucleus and nucleolus, where it regulates pre-rRNA processing and ribosome maturation.
PUM3 contains eight PUF RNA-binding domains arranged in a crescent-shaped architecture that recognizes conserved 5'-UGUANAUA-3' motifs in RNA targets. It binds ribosomal RNA precursors and ribosome assembly factors, influencing the processing of 28S and 5.8S rRNA. Depletion of PUM3 results in rRNA maturation defects, altered nucleolar morphology, and reduced ribosome production. Beyond ribosome biogenesis, PUM3 may also modulate mRNA stability and translation through interactions with additional RNA-binding proteins and components of the exosome complex.
The PUM3 antibody is used in molecular biology, RNA regulation, and ribosome assembly research to detect Pumilio homolog 3 protein and analyze its cellular localization. Western blot analysis identifies a 74 kilodalton band, while immunofluorescence reveals prominent nucleolar staining overlapping with fibrillarin. Because of its role in nucleolar RNA metabolism, this antibody provides a tool to study gene expression regulation at the post-transcriptional level.
Recent studies suggest that PUM3 interacts with factors controlling RNA stability and surveillance, positioning it as a regulator of RNA quality control. The PUM3 antibody facilitates the exploration of these processes and supports studies of nucleolar function, gene expression, and RNA-protein network organization. NSJ Bioreagents provides this antibody validated for its applications, ensuring specificity and reliability for RNA metabolism research. -
UniProt
- Q15397
抗原
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