PIF1 抗体 (AA 31-618)
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北京 101111
Quick Overview for PIF1 抗体 (AA 31-618) (ABIN7873798)
抗原
See all PIF1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 31-618
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原理
- PIF1 Antibody / ATP-dependent DNA helicase PIF1
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human PIF1 recombinant protein (Position: Q31-R618) was used as the immunogen for the PIF1 antibody.
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亚型
- IgG
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anti-PIF1 5'-To-3' DNA Helicase (PIF1) (Internal Region) antibody
PIF1 适用: 人, 小鼠, 大鼠 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-PIF1 5'-To-3' DNA Helicase (PIF1) (AA 125-360) antibodyPIF1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-PIF1 5'-To-3' DNA Helicase (PIF1) antibodyPIF1 适用: 人, 小鼠 ELISA, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the PIF1 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the PIF1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- PIF1 (PIF1 5'-To-3' DNA Helicase (PIF1))
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别名
- PIF1
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背景
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PIF1 antibody detects ATP-dependent DNA helicase PIF1, encoded by the PIF1 gene on chromosome 15q24. PIF1 antibody is widely used to study this multifunctional helicase, which unwinds DNA in both nuclear and mitochondrial compartments. PIF1 belongs to the RecD subfamily of helicases and is essential for maintaining genome stability by resolving DNA secondary structures, promoting telomere maintenance, and facilitating replication fork progression. It uses ATP hydrolysis to unwind duplex DNA and has a strong preference for G-rich sequences capable of forming G-quadruplex structures.
Structurally, PIF1 contains a conserved helicase domain with Walker A and B motifs for ATP binding and hydrolysis, as well as DNA-binding domains that allow recognition of G-quadruplexes. PIF1 is highly conserved from yeast to humans, underscoring its fundamental role in DNA metabolism. Alternative splicing produces isoforms targeted to the nucleus and mitochondria, allowing PIF1 to function in both genomic and mitochondrial DNA maintenance.
Functionally, PIF1 contributes to telomere length regulation by displacing telomerase from DNA ends, preventing uncontrolled elongation. It also suppresses genome instability by unwinding secondary DNA structures at replication forks and transcription units. In mitochondria, PIF1 ensures faithful replication and repair of mtDNA, protecting against deletions and rearrangements. Knockdown or mutation of PIF1 leads to increased DNA damage, replication stress, and mitochondrial dysfunction. Researchers use PIF1 antibody to explore DNA replication, telomere biology, and genome integrity mechanisms.
Clinically, PIF1 mutations are associated with cancer predisposition and mitochondrial disease. Rare germline variants have been linked to increased risk of breast cancer, while somatic mutations are detected in diverse tumor types. Loss of PIF1 function contributes to mitochondrial genome instability, which underlies certain neuromuscular disorders. PIF1's role in suppressing G-quadruplexes also makes it relevant to anticancer therapies, as stabilization of these structures can selectively kill tumor cells. NSJ Bioreagents provides PIF1 antibody as a validated reagent for cancer biology, telomere studies, and mitochondrial research.
Experimentally, PIF1 antibody is applied in western blotting to detect isoforms ranging from ~70-80 kDa, in immunofluorescence microscopy to study nuclear and mitochondrial localization, and in immunoprecipitation assays to isolate DNA repair complexes. These applications highlight its utility in analyzing DNA helicase activity and genome maintenance pathways. -
UniProt
- Q9H611
抗原
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