GLRX3 抗体 (AA 31-307)
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Quick Overview for GLRX3 抗体 (AA 31-307) (ABIN7873780)
抗原
See all GLRX3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 31-307
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原理
- GLRX3 Antibody / Glutaredoxin 3 / PICOT
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human GLRX3 recombinant protein (Position: K31-V307) was used as the immunogen for the GLRX3 antibody.
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亚型
- IgG
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anti-Glutaredoxin 3 (GLRX3) (AA 73-320) antibody
GLRX3 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutaredoxin 3 (GLRX3) (AA 81-110), (N-Term) antibodyGLRX3 适用: 人, 小鼠 WB, FACS 宿主: 兔 Polyclonal RB20866 unconjugated
anti-Glutaredoxin 3 (GLRX3) (AA 1-335) antibodyGLRX3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4B5-2A8 unconjugated
anti-Glutaredoxin 3 (GLRX3) (AA 1-335) antibodyGLRX3 适用: 人 WB, IHC, IP, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Glutaredoxin 3 (GLRX3) antibodyKD Validated GLRX3 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 24GB13145 unconjugated
anti-Glutaredoxin 3 (GLRX3) (AA 1-335) antibodyGLRX3 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Glutaredoxin 3 (GLRX3) (AA 81-110) antibodyGLRX3 适用: 人, 小鼠 WB, IHC, FACS, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glutaredoxin 3 (GLRX3) (AA 144-234) antibodyGLRX3 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutaredoxin 3 (GLRX3) (AA 10-117) antibodyGLRX3 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutaredoxin 3 (GLRX3) (N-Term) antibodyGLRX3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the GLRX3 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the GLRX3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- GLRX3 (Glutaredoxin 3 (GLRX3))
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别名
- GLRX3
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背景
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GLRX3 antibody detects Glutaredoxin-3 (also known as PICOT or Thioredoxin-like protein 2), a multifunctional redox enzyme that regulates cellular redox homeostasis, iron-sulfur cluster assembly, and signal transduction. Encoded by the GLRX3 gene on chromosome 10q26.3, this protein belongs to the glutaredoxin family, characterized by their thiol-disulfide oxidoreductase activity. GLRX3 contains two monothiol glutaredoxin domains that utilize glutathione to catalyze reversible thiol exchange reactions, thereby maintaining protein thiol status and redox balance. Beyond redox control, GLRX3 acts as a scaffold protein for Fe-S cluster delivery and as a modulator of intracellular signaling cascades.
GLRX3 is ubiquitously expressed but particularly abundant in heart, skeletal muscle, and liver, where it contributes to mitochondrial function and oxidative stress resistance. The protein forms complexes with BolA-like proteins to facilitate Fe-S cluster biogenesis, which is essential for electron transport chain integrity. In cardiomyocytes, GLRX3i 1/2originally identified as PICOT (PKC-interacting cousin of thioredoxin)i 1/2binds to protein kinase C theta and inhibits its activity, modulating hypertrophic signaling and contractility. This redox-regulatory interaction links GLRX3 to cardiac protection against oxidative damage and maladaptive stress responses.
The GLRX3 antibody is widely used in redox biology, metabolism, and cardiovascular research to examine oxidative stress regulation, Fe-S cluster metabolism, and cell signaling. Western blot analysis identifies a 38 kilodalton band corresponding to GLRX3, while immunofluorescence shows cytoplasmic and mitochondrial localization. Studies using the antibody have revealed that GLRX3 depletion leads to increased ROS levels, impaired mitochondrial respiration, and reduced iron homeostasis. Because these effects contribute to cellular senescence and disease progression, GLRX3 is of growing interest as a therapeutic target in oxidative and metabolic disorders.
Beyond its roles in redox and energy metabolism, GLRX3 contributes to developmental processes and immune function. It modulates NF-?B and MAPK pathways, influencing inflammatory gene expression. Overexpression has been reported in cancers such as hepatocellular carcinoma and breast carcinoma, where it promotes proliferation and resistance to oxidative stress. The GLRX3 antibody therefore provides a valuable reagent for investigating molecular mechanisms of redox regulation, signal transduction, and disease adaptation. NSJ Bioreagents supplies this antibody validated for its applications, ensuring high specificity and reproducibility for both basic and translational research applications. -
UniProt
- O76003
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途径
- Cell RedoxHomeostasis
抗原
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