Nidogen 1 抗体 (AA 31-1209)
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Quick Overview for Nidogen 1 抗体 (AA 31-1209) (ABIN7873746)
抗原
See all Nidogen 1 (NID1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 31-1209
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原理
- NID1 Antibody / Nidogen 1 / Entactin
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human Entactin/NID1 recombinant protein (Position: R31-H1209) was used as the immunogen for the NID1 antibody.
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亚型
- IgG
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anti-Nidogen 1 (NID1) (AA 1-1114) antibody
NID1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Nidogen 1 (NID1) (AA 1148-1247) antibodyNID1 适用: 人 ELISA, WB 宿主: 小鼠 Monoclonal 1G3 unconjugated
anti-Nidogen 1 (NID1) (AA 1-1114) antibodyNID1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Nidogen 1 (NID1) (Internal Region) antibodyNID1 适用: 人 ELISA, WB, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Nidogen 1 (NID1) antibodyNID1 适用: 小鼠 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nidogen 1 (NID1) antibodyNID1 适用: 小鼠, 人 ELISA, WB, BI (rec-lig) 宿主: 大鼠 Monoclonal JF2 unconjugated
anti-Nidogen 1 (NID1) antibodyNID1 适用: 人 WB, FACS 宿主: 兔 Monoclonal 24GB4995 unconjugated Recombinant Antibody
anti-Nidogen 1 (NID1) (AA 428-665) antibodyNID1 适用: 小鼠 ELISA, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the NID1 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the NID1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- Nidogen 1 (NID1)
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别名
- NID1
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背景
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NID1 antibody is used to study Nidogen 1, also known as Entactin, a key basement membrane linker that bridges laminin networks with collagen IV scaffolds to establish tissue architecture and mechanical stability. As a secreted glycoprotein with multiple binding modules, Nidogen 1 connects laminin gamma chains to collagen IV and perlecan, organizing a resilient sheet that supports epithelial polarity, filtration barriers, and neuromuscular junctions. Through these interactions, the protein shapes morphogenesis, angiogenesis, and repair, and it helps buffer tissues against mechanical stress.
Nidogen 1 contains globular domains separated by flexible segments that adapt to different extracellular partners. Its localization along the basement membrane enables crosstalk between matrix and cells, influencing receptor signaling through integrins and dystroglycan. Depletion in animal models disrupts organogenesis and compromises barrier function in kidney and vasculature, highlighting the protein's structural and signaling roles. Because matrix composition tunes growth factor availability, Nidogen 1 also contributes to gradient formation and ligand presentation within tissue microenvironments.
Researchers use NID1 antibody / Entactin in immunohistochemistry to delineate basement membranes in skin, kidney, lung, and tumor samples, providing clear borders for morphometric analyses and pathology scoring. In immunoblotting, the reagent tracks expression during development or matrix remodeling, while immunofluorescence co-localization with laminin and collagen IV reveals network integrity and continuity. In vitro, combining the antibody with matrix assembly assays clarifies how Nidogen 1 scaffolds influence cell adhesion, migration, and polarity under defined conditions.
The NID1 antibody / Entactin from NSJ Bioreagents performs reliably in tissue sections and cultured cells, enabling teams to standardize basement membrane visualization across experiments and sample types. By pairing matrix mapping with transcriptomics or proteomics, investigators can connect changes in Nidogen 1 to shifts in epithelial organization, vascular stability, and tumor-stroma interplay, thereby linking structural cues to functional outcomes in both physiology and disease. -
UniProt
- P14543
抗原
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