SPIDR 抗体 (AA 273-904)
The 兔 多克隆 anti-SPIDR antibody is suitable to detect SPIDR in samples from 人, 小鼠 和 大鼠. It has been validated for WB, ELISA, FACS, ICC 和 IF.
产品编号 ABIN7873197
发货至:
中国
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Quick Overview for SPIDR 抗体 (AA 273-904) (ABIN7873197)
抗原
SPIDR
(Scaffolding Protein Involved in DNA Repair (SPIDR))
适用
人, 小鼠, 大鼠
宿主
兔
克隆类型
多克隆
标记
This SPIDR antibody is un-conjugated
应用范围
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
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抗原表位
- AA 273-904
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原理
- SPIDR Antibody / Scaffold protein involved in DNA repair
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human SPIDR recombinant protein (Position: E273-E904) was used as the immunogen for the SPIDR antibody.
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亚型
- IgG
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anti-Scaffolding Protein Involved in DNA Repair (SPIDR) (AA 1-915) antibody
SPIDR 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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应用备注
- Optimal dilution of the SPIDR antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the SPIDR antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- SPIDR (Scaffolding Protein Involved in DNA Repair (SPIDR))
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别名
- SPIDR
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背景
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SPIDR antibody detects Scaffold protein involved in DNA repair (also known as KIAA0146), a nuclear adaptor protein that coordinates homologous recombination and DNA double-strand break repair. Encoded by the SPIDR gene on chromosome 8q24.3, this protein functions as a scaffold linking key repair enzymes including RAD51, BRCA2, and RPA to the DNA damage response machinery. SPIDR facilitates the assembly of repair complexes at damaged DNA sites and promotes strand invasion and exchange during homologous recombination.
SPIDR contains multiple interaction domains, including coiled-coil regions and binding motifs that mediate association with RAD51 paralogs and BRCA2. Following DNA damage, SPIDR localizes to nuclear foci corresponding to double-strand break sites, where it promotes RAD51 filament formation and repair fidelity. By stabilizing repair intermediates, SPIDR ensures proper resolution of recombination events and maintenance of genomic stability. Depletion of SPIDR impairs DNA repair efficiency and increases chromosomal aberrations, underscoring its importance in genome maintenance.
The SPIDR antibody is used in DNA repair, cancer, and cell cycle research to analyze homologous recombination pathways and genomic stability. Western blot analysis identifies a 90 kilodalton band corresponding to SPIDR, while immunofluorescence shows punctate nuclear foci following genotoxic stress. This antibody enables researchers to track DNA repair factor recruitment and assess homologous recombination efficiency.
Defects in SPIDR expression or localization are associated with hypersensitivity to DNA-damaging agents and increased genomic instability, contributing to cancer susceptibility. SPIDR also cooperates with checkpoint proteins such as ATR and CHK1 to coordinate DNA repair with replication stress responses. The SPIDR antibody supports these investigations by providing specific detection in western blotting, flow cytometry, and immunofluorescence applications. NSJ Bioreagents validates this antibody for use in research on DNA repair dynamics, chromatin remodeling, and tumor suppressor pathways. -
UniProt
- Q14159
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途径
- Positive Regulation of Response to DNA Damage Stimulus
抗原
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