SH2B1 抗体 (AA 26-715)
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北京 101111
Quick Overview for SH2B1 抗体 (AA 26-715) (ABIN7873014)
抗原
See all SH2B1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 26-715
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原理
- SH2B1 Antibody / SH2B adapter protein 1
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human SH2B1 recombinant protein (Position: W26-Q715) was used as the immunogen for the SH2B1 antibody.
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亚型
- IgG
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anti-SH2B Adaptor Protein 1 (SH2B1) antibody
SH2B1 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-SH2B Adaptor Protein 1 (SH2B1) (AA 1-426) antibodySH2B1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-SH2B Adaptor Protein 1 (SH2B1) (AA 327-426) antibodySH2B1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2B9 unconjugated
anti-SH2B Adaptor Protein 1 (SH2B1) (Internal Region) antibodySH2B1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-SH2B Adaptor Protein 1 (SH2B1) (AA 395-444) antibodySH2B1 适用: 人, 小鼠, 大鼠, 犬, 豚鼠, 马, 兔, Bat, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-SH2B Adaptor Protein 1 (SH2B1) (AA 360-520) antibodySH2B1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-SH2B Adaptor Protein 1 (SH2B1) (AA 1-426) antibodySH2B1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-SH2B Adaptor Protein 1 (SH2B1) antibodySH2B1 适用: 人, 小鼠, 大鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the SH2B1 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the SH2B1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- SH2B1 (SH2B Adaptor Protein 1 (SH2B1))
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别名
- SH2B1
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背景
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SH2B1 antibody detects SH2B adapter protein 1, a signaling adaptor that regulates insulin, leptin, and growth factor receptor pathways controlling metabolism and energy balance. Encoded by the SH2B1 gene on chromosome 16p11.2, this cytoplasmic adaptor belongs to the SH2B family of signal transduction proteins and serves as a key modulator of receptor tyrosine kinase and cytokine receptor signaling. SH2B1 enhances insulin receptor signaling, JAK2 activation, and downstream PI3K and MAPK pathways involved in glucose metabolism, cell growth, and neuronal signaling.
Structurally, SH2B1 is a 756-amino-acid protein of approximately 85 kilodaltons containing an N-terminal dimerization domain, a central pleckstrin homology (PH) domain, and a C-terminal Src homology 2 (SH2) domain that mediates phosphotyrosine-dependent interactions. It exists in multiple isoforms (alpha, beta, gamma, and delta) produced by alternative splicing, with tissue-specific expression across brain, liver, adipose tissue, and skeletal muscle. The protein acts as a scaffold that bridges receptor kinases to intracellular effectors, amplifying or modulating signaling intensity and duration.
The SH2B1 antibody is widely used in metabolism, endocrinology, and neurobiology research to study insulin signaling, leptin sensitivity, and receptor-mediated pathways regulating energy homeostasis. Western blot analysis detects a band of approximately 85 kilodaltons corresponding to SH2B1, while immunofluorescence reveals cytoplasmic and plasma membrane localization. This antibody is an essential reagent for exploring the molecular basis of metabolic signaling and neuronal regulation.
Functionally, SH2B1 binds to insulin and leptin receptors through JAK2 and IRS1, enhancing phosphorylation cascades that regulate glucose uptake and appetite control. Loss or mutation of SH2B1 disrupts these pathways, leading to insulin resistance, obesity, and metabolic syndrome. Deletions encompassing SH2B1 are associated with severe early-onset obesity and neurobehavioral abnormalities, while overexpression can increase leptin sensitivity and improve glucose tolerance. Beyond metabolism, SH2B1 contributes to neuronal growth, regeneration, and synaptic plasticity through its modulation of NGF and BDNF signaling. The SH2B1 antibody provides a robust tool for studying these signaling mechanisms and their implications in metabolic and neurological disorders. NSJ Bioreagents validates this antibody for its applications, ensuring specific and reproducible results across biological systems. -
UniProt
- Q9NRF2
抗原
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