NAGLU 抗体 (AA 24-489)
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Quick Overview for NAGLU 抗体 (AA 24-489) (ABIN7872697)
抗原
See all NAGLU 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 24-489
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原理
- NAGLU Antibody / Alpha-N-acetylglucosaminidase
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human NAGLU recombinant protein (Position: D24-D489) was used as the immunogen for the NAGLU antibody.
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亚型
- IgG
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anti-N-Acetylglucosaminidase, alpha (NAGLU) (AA 485-743) antibody
NAGLU 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-N-Acetylglucosaminidase, alpha (NAGLU) (AA 644-742) antibodyNAGLU 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1B7 unconjugated
anti-N-Acetylglucosaminidase, alpha (NAGLU) (Center) antibodyNAGLU 适用: 人 WB, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-N-Acetylglucosaminidase, alpha (NAGLU) (AA 644-742) antibodyNAGLU 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-N-Acetylglucosaminidase, alpha (NAGLU) (AA 429-710) antibodyNAGLU 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-N-Acetylglucosaminidase, alpha (NAGLU) (AA 449-709) antibodyNAGLU 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the NAGLU antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the NAGLU antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- NAGLU (N-Acetylglucosaminidase, alpha (NAGLU))
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别名
- NAGLU
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背景
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NAGLU antibody detects Alpha-N-acetylglucosaminidase, a lysosomal enzyme responsible for the degradation of heparan sulfate. Encoded by the NAGLU gene on chromosome 17q21.2, this hydrolase catalyzes the removal of N-acetylglucosamine residues from the non-reducing ends of heparan sulfate chains, playing a critical role in glycosaminoglycan catabolism. Proper function of NAGLU is essential for maintaining lysosomal homeostasis and preventing the accumulation of undegraded substrates.
NAGLU is synthesized as a preproenzyme that undergoes proteolytic processing in the endoplasmic reticulum and Golgi before trafficking to lysosomes. The mature enzyme is approximately 83 kilodaltons and operates optimally at acidic pH . It functions in concert with other lysosomal hydrolases to complete stepwise degradation of heparan sulfate, ensuring normal turnover of extracellular matrix components and cell-surface proteoglycans.
The NAGLU antibody is widely used in enzymology, lysosomal biology, and metabolic disease research to study glycosaminoglycan metabolism, lysosomal trafficking, and enzyme replacement mechanisms. Western blot analysis identifies a band corresponding to the mature lysosomal form of NAGLU, while immunofluorescence shows vesicular staining overlapping with lysosomal markers such as LAMP1. This antibody enables precise detection of enzyme localization and expression changes in normal and diseased tissues.
Mutations in NAGLU cause mucopolysaccharidosis type IIIB (MPS IIIB), also known as Sanfilippo syndrome type B, a lysosomal storage disorder characterized by progressive neurodegeneration and systemic accumulation of heparan sulfate. The NAGLU antibody is a valuable tool for diagnosing and modeling this disease, assessing enzyme replacement efficacy, and monitoring lysosomal function in gene therapy studies. NSJ Bioreagents provides this antibody validated for its applications, ensuring high sensitivity for lysosomal and metabolic research. -
UniProt
- P54802
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途径
- Glycosaminoglycan Metabolic Process
抗原
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