KIAA0090 抗体 (AA 23-993)
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Quick Overview for KIAA0090 抗体 (AA 23-993) (ABIN7872559)
抗原
See all KIAA0090 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 23-993
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原理
- EMC1 Antibody / ER membrane protein complex subunit 1
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human KIAA0090/EMC1 recombinant protein (Position: Y23-R993) was used as the immunogen for the EMC1 antibody.
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亚型
- IgG
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anti-KIAA0090 (KIAA0090) (AA 866-894), (C-Term) antibody
KIAA0090 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB25008 unconjugated
anti-KIAA0090 (KIAA0090) (AA 873-902), (C-Term) antibodyKIAA0090 适用: 人, 小鼠 WB, FACS, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-KIAA0090 (KIAA0090) antibodyKIAA0090 适用: 人, 小鼠, 大鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-KIAA0090 (KIAA0090) (AA 875-925) antibodyKIAA0090 适用: 人, 小鼠 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-KIAA0090 (KIAA0090) (AA 1-50) antibodyKIAA0090 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-KIAA0090 (KIAA0090) (AA 885-965) antibodyKIAA0090 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the EMC1 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the EMC1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- KIAA0090
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别名
- EMC1
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背景
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EMC1 antibody detects ER membrane protein complex subunit 1, a structural component of the ER membrane protein complex (EMC) responsible for folding and insertion of transmembrane proteins. EMC1 acts as a scaffold stabilizing the entire complex and supports proper biogenesis of multipass membrane proteins. The EMC1 antibody is used in cell biology, proteostasis, and neurodevelopmental research to study membrane protein maturation and ER quality control.
EMC1 is encoded by the EMC1 gene located on human chromosome 1p36.13. The protein is approximately 993 amino acids long and localizes to the endoplasmic reticulum membrane, where it forms part of a 10-subunit complex including EMC2 through EMC10. EMC1 contains multiple transmembrane segments and luminal domains that interact with nascent polypeptides emerging from the ribosome-translocon system.
The EMC1 antibody detects a 115 kilodalton band by western blot and shows reticular ER staining under immunofluorescence microscopy. EMC1 facilitates the co-translational insertion and folding of membrane proteins, including G-protein coupled receptors, ion channels, and transporters. It also interacts with the ER-associated degradation (ERAD) machinery to remove misfolded proteins and prevent aggregation.
Mutations in EMC1 lead to congenital disorders involving neurodevelopmental delay, cerebellar atrophy, and visual impairment, highlighting its importance in neuronal function. In model systems, EMC1 loss disrupts ER morphology and mitochondrial contacts, leading to stress responses and apoptosis. EMC1 also contributes to cholesterol biosynthesis by ensuring proper insertion of enzymes involved in lipid metabolism.
Through its essential role in membrane protein biogenesis and ER stability, EMC1 is a cornerstone of cellular proteostasis. NSJ Bioreagents provides a validated EMC1 antibody optimized for its applications, supporting studies of protein folding, ER homeostasis, and disease-associated misfolding mechanisms. -
UniProt
- Q8N766
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途径
- SARS-CoV-2 Protein Interactome
抗原
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