POMT1 抗体 (AA 224-747)
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Quick Overview for POMT1 抗体 (AA 224-747) (ABIN7872396)
抗原
See all POMT1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 224-747
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原理
- POMT1 Antibody / Protein O-mannosyl-transferase 1
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human POMT1 recombinant protein (Position: H224-H747) was used as the immunogen for the POMT1 antibody.
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亚型
- IgG
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anti-Protein-O-Mannosyltransferase 1 (POMT1) (AA 706-735), (C-Term) antibody
POMT1 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB31305 unconjugated
anti-Protein-O-Mannosyltransferase 1 (POMT1) (AA 713-743), (C-Term) antibodyPOMT1 适用: 人 WB, FACS, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Protein-O-Mannosyltransferase 1 (POMT1) (AA 318-513) antibodyPOMT1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein-O-Mannosyltransferase 1 (POMT1) (AA 706-735), (C-Term) antibodyPOMT1 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Protein-O-Mannosyltransferase 1 (POMT1) (AA 310-550) antibodyPOMT1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Protein-O-Mannosyltransferase 1 (POMT1) (AA 648-697) antibodyPOMT1 适用: 人, 小鼠, 大鼠, Cow, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Protein-O-Mannosyltransferase 1 (POMT1) (AA 318-513) antibodyPOMT1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the POMT1 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the POMT1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- POMT1 (Protein-O-Mannosyltransferase 1 (POMT1))
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别名
- POMT1
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背景
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POMT1 antibody detects Protein O-mannosyl-transferase 1, an enzyme required for the initial step of O-mannosyl glycan biosynthesis on glycoproteins, particularly alpha-dystroglycan. The UniProt recommended name is Protein O-mannosyl-transferase 1 (POMT1). This enzyme catalyzes the transfer of mannose from dolichol phosphate-mannose to serine or threonine residues on target proteins, a process essential for proper protein folding, stability, and function.
Functionally, POMT1 antibody identifies a 747-amino-acid endoplasmic reticulum (ER) membrane protein that forms a functional heterodimer with POMT2. This complex initiates the O-mannosyl glycosylation pathway, which is critical for dystroglycan processing and muscle integrity. POMT1 ensures the attachment of the first mannose residue, forming the foundation for subsequent glycan extensions that link the extracellular matrix to the cytoskeleton via alpha-dystroglycan.
The POMT1 gene is located on chromosome 9q34.13 and is expressed in brain, skeletal muscle, and heart. It plays a vital role in muscle development, neuronal migration, and synaptic organization by supporting glycosylation-dependent cell adhesion and signaling.
Pathologically, mutations in POMT1 cause congenital muscular dystrophy-dystroglycanopathy type A1 (Walker-Warburg syndrome), type B1, and type C1. These disorders are characterized by muscular dystrophy, brain malformations, and ocular defects due to defective O-mannosylation of dystroglycan. Research using POMT1 antibody supports studies in glycosylation pathways, muscular dystrophy, and neuronal development.
POMT1 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect ER glycosyltransferases. NSJ Bioreagents provides POMT1 antibody reagents optimized for studies in protein modification, muscle biology, and glycoprotein processing.
Structurally, Protein O-mannosyl-transferase 1 contains multiple transmembrane domains and a luminal catalytic domain harboring the conserved DXD motif required for glycosyltransferase activity. It assembles with POMT2 to form an enzymatically active complex that determines substrate recognition and catalysis. This antibody aids in investigating POMT1i 1/2s role in glycoprotein biosynthesis, muscle development, and congenital disease mechanisms. -
UniProt
- Q9Y6A1
抗原
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