SIGLEC15 抗体 (AA 22-319)
This 兔 多克隆 antibody specifically detects SIGLEC15 in ELISA, WB, FACS, IF 和 ICC. It exhibits reactivity toward 人.
产品编号 ABIN7872317
发货至:
中国
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Quick Overview for SIGLEC15 抗体 (AA 22-319) (ABIN7872317)
抗原
See all SIGLEC15 抗体
SIGLEC15
(Sialic Acid Binding Ig-Like Lectin 15 (SIGLEC15))
适用
人
宿主
兔
克隆类型
多克隆
标记
This SIGLEC15 antibody is un-conjugated
应用范围
ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)
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抗原表位
- AA 22-319
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原理
- SIGLEC15 Antibody / Sialic acid-binding Ig-like lectin 15
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human SIGLEC15 recombinant protein (Position: R22-R319) was used as the immunogen for the SIGLEC15 antibody.
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亚型
- IgG
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anti-Sialic Acid Binding Ig-Like Lectin 15 (SIGLEC15) (AA 274-302), (C-Term) antibody
SIGLEC15 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB29353 unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 15 (SIGLEC15) (C-Term) antibodySIGLEC15 适用: 人 WB 宿主: 兔 Monoclonal BLR276L unconjugated Recombinant Antibody
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应用备注
- Optimal dilution of the SIGLEC15 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the SIGLEC15 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- SIGLEC15 (Sialic Acid Binding Ig-Like Lectin 15 (SIGLEC15))
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别名
- SIGLEC15
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背景
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SIGLEC15 antibody recognizes Sialic acid-binding Ig-like lectin 15, a type I transmembrane protein belonging to the Siglec (sialic acid-binding immunoglobulin-like lectins) family of immune modulators. SIGLEC15 plays an important role in immune regulation, osteoclast differentiation, and tumor immune evasion. It binds sialylated glycoconjugates on target cells, transmitting inhibitory or activating signals that shape immune responses. The SIGLEC15 antibody is widely used in immunology, oncology, and bone biology research to study checkpoint signaling and macrophage-mediated immune regulation.
Sialic acid-binding Ig-like lectin 15 is encoded by the SIGLEC15 gene located on human chromosome 18q12.3. The protein consists of an extracellular N-terminal V-set immunoglobulin domain that recognizes sialylated glycans, a transmembrane segment, and a short cytoplasmic tail that associates with adaptor proteins such as DAP12 or DAP10 to mediate downstream signaling. In osteoclasts, SIGLEC15 promotes differentiation and bone resorption by activating phosphoinositide 3-kinase and mitogen-activated protein kinase cascades. In immune cells, it functions as a checkpoint ligand that suppresses T-cell activation, analogous to PD-L1.
Studies using the SIGLEC15 antibody have shown that this protein is upregulated on tumor-associated macrophages and osteoclasts in various malignancies and inflammatory diseases. Western blot analyses typically detect a 35-40 kilodalton glycoprotein band. Immunohistochemistry demonstrates strong membrane staining in macrophage-rich regions of tumors and in bone-resorptive zones. Functionally, SIGLEC15 acts as an immune suppressor by binding unidentified receptors on T cells and natural killer cells, inhibiting cytokine production and cytotoxic responses. Its expression inversely correlates with PD-L1, suggesting a complementary role in immune evasion mechanisms exploited by tumors.
Therapeutic targeting of SIGLEC15 has emerged as a promising strategy in cancer immunotherapy, particularly for patients unresponsive to PD-1/PD-L1 blockade. The protein also regulates osteoclast activity and bone remodeling, making it relevant in osteoporosis and arthritis research. NSJ Bioreagents provides a validated SIGLEC15 antibody optimized for western blot, flow cytometry, and immunohistochemistry. This reagent enables researchers to investigate innate immune regulation, tumor microenvironment biology, and checkpoint-based immunotherapy development. -
UniProt
- Q6ZMC9
抗原
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