PIK3C2A 抗体 (AA 22-1523)
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Quick Overview for PIK3C2A 抗体 (AA 22-1523) (ABIN7872277)
抗原
See all PIK3C2A 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 22-1523
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原理
- PIK3C2A Antibody / Phosphoinositide 3-kinase-C2-alpha
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human PIK3C2A recombinant protein (Position: R22-E1523) was used as the immunogen for the PIK3C2A antibody.
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亚型
- IgG
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anti-Phosphoinositide-3-Kinase, Class 2, alpha Polypeptide (PIK3C2A) (AA 172-403) antibody
PIK3C2A 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Phosphoinositide-3-Kinase, Class 2, alpha Polypeptide (PIK3C2A) (AA 1577-1686) antibodyPIK3C2A 适用: 人 WB, ELISA, IHC (p), IF 宿主: 小鼠 Monoclonal 3E7 unconjugated
anti-Phosphoinositide-3-Kinase, Class 2, alpha Polypeptide (PIK3C2A) (C-Term) antibodyVerified PIK3C2A 适用: 人 IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Phosphoinositide-3-Kinase, Class 2, alpha Polypeptide (PIK3C2A) antibodyKD Validated PIK3C2A 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 24GB14050 unconjugated
anti-Phosphoinositide-3-Kinase, Class 2, alpha Polypeptide (PIK3C2A) (AA 1577-1687) antibodyPIK3C2A 适用: 人 WB, IHC, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3E7 unconjugated
anti-Phosphoinositide-3-Kinase, Class 2, alpha Polypeptide (PIK3C2A) (C-Term) antibodyPIK3C2A 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Phosphoinositide-3-Kinase, Class 2, alpha Polypeptide (PIK3C2A) (AA 349-611) antibodyPIK3C2A 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the PIK3C2A antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the PIK3C2A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- PIK3C2A (Phosphoinositide-3-Kinase, Class 2, alpha Polypeptide (PIK3C2A))
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别名
- PIK3C2A
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背景
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PIK3C2A antibody detects Phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing alpha polypeptide, a lipid kinase that regulates membrane trafficking, signal transduction, and cytoskeletal organization. Encoded by the PIK3C2A gene on chromosome 11p15.5, this enzyme belongs to the class II phosphoinositide 3-kinase (PI3K) family and phosphorylates phosphatidylinositol and phosphatidylinositol 4-phosphate to generate phosphatidylinositol 3-phosphate (PI3P) and phosphatidylinositol 3,4-bisphosphate (PI(3,4)P2). Through these lipid intermediates, PIK3C2A coordinates endocytosis, vesicle trafficking, and cell polarity regulation.
PIK3C2A contains a catalytic kinase domain, a C2 lipid-binding domain, and clathrin-binding regions that localize it to the plasma membrane and endosomes. Its enzymatic activity is essential for clathrin-mediated endocytosis and autophagosome formation. PIK3C2A also modulates signaling pathways downstream of receptor tyrosine kinases and integrins by influencing AKT activation and cytoskeletal reorganization.
The PIK3C2A antibody is widely used in cell signaling, membrane biology, and cancer research to study phosphoinositide metabolism, vesicle formation, and kinase regulation. Western blot analysis identifies a 180-190 kilodalton band corresponding to PIK3C2A, while immunofluorescence reveals punctate staining in endosomal and plasma membrane compartments. This antibody enables the analysis of lipid signaling networks that control intracellular transport and cellular morphology.
Loss or mutation of PIK3C2A has been linked to congenital cataracts, vascular defects, and impaired cilia formation due to disrupted membrane trafficking. Conversely, overexpression contributes to tumorigenesis through altered PI3K signaling and enhanced migration. The PIK3C2A antibody provides a robust tool for investigating lipid kinase function in physiology and disease. NSJ Bioreagents validates this antibody for western blotting and immunofluorescence, ensuring accuracy for research into phosphoinositide and trafficking pathways. -
UniProt
- O00443
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途径
- EGFR Signaling Pathway, Inositol Metabolic Process, Platelet-derived growth Factor Receptor Signaling
抗原
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