TRIM32 抗体 (AA 216-586)
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Quick Overview for TRIM32 抗体 (AA 216-586) (ABIN7872242)
抗原
See all TRIM32 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 216-586
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原理
- TRIM32 Antibody / Tripartite motif-containing protein 32
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human TRIM32 recombinant protein (Position: S216-R586) was used as the immunogen for the TRIM32 antibody.
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亚型
- IgG
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anti-Tripartite Motif Containing 32 (TRIM32) (AA 105-204) antibody
TRIM32 适用: 人 WB, ELISA, IF, RNAi 宿主: 小鼠 Monoclonal 2E5 unconjugated
anti-Tripartite Motif Containing 32 (TRIM32) (C-Term) antibodyTRIM32 适用: 人, 小鼠, 大鼠, 犬, Cow, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Tripartite Motif Containing 32 (TRIM32) antibodyTRIM32 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Tripartite Motif Containing 32 (TRIM32) (AA 105-204) antibodyTRIM32 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Tripartite Motif Containing 32 (TRIM32) (AA 549-598) antibodyTRIM32 适用: 人, 小鼠, 大鼠, 犬, Cow, 马, 兔, 豚鼠, 斑马鱼, Pig, 小鸡, 猴, 非洲爪蟾 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Tripartite Motif Containing 32 (TRIM32) (AA 559-608) antibodyTRIM32 适用: 人, 小鼠, 大鼠, 犬, Cow, 马, 兔, 豚鼠, 斑马鱼, Pig, 小鸡, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Tripartite Motif Containing 32 (TRIM32) (AA 1-280) antibodyTRIM32 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Tripartite Motif Containing 32 (TRIM32) (C-Term) antibodyTRIM32 适用: 人, 小鼠, 大鼠, 犬, Cow, 马, 兔, 豚鼠, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Tripartite Motif Containing 32 (TRIM32) (AA 301-400) antibodyTRIM32 适用: 小鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Tripartite Motif Containing 32 (TRIM32) (AA 1-653) antibodyTRIM32 适用: 人 IP 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the TRIM32 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the TRIM32 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- TRIM32 (Tripartite Motif Containing 32 (TRIM32))
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别名
- TRIM32
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背景
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TRIM32 antibody detects Tripartite motif-containing protein 32, an E3 ubiquitin ligase that regulates protein turnover, muscle differentiation, and cellular signaling. TRIM32 is part of the TRIM family of RING finger proteins that mediate ubiquitin-dependent degradation and transcriptional regulation. The TRIM32 antibody is widely used in muscle biology, neurodegeneration, and oncology research to study ubiquitination, differentiation, and signaling regulation.
TRIM32 is encoded by the TRIM32 gene located on human chromosome 9q33.1. The protein is approximately 653 amino acids long and contains a RING domain, one or two B-box domains, and a coiled-coil region that mediates protein-protein interactions, followed by a C-terminal NHL repeat domain responsible for substrate recognition. TRIM32 localizes to the cytoplasm, nucleus, and occasionally to cytoskeletal structures, depending on cell type and activity state.
The TRIM32 antibody detects an 80 kilodalton protein by western blot and shows punctate cytoplasmic staining under immunofluorescence microscopy. TRIM32 functions as an E3 ubiquitin ligase that targets a variety of substrates involved in differentiation, including actin regulators, transcription factors, and signaling molecules. In skeletal muscle, TRIM32 promotes myogenesis by ubiquitinating inhibitors of differentiation such as NDRG2 and MyoD regulators. In neurons, TRIM32 modulates synaptic plasticity and axonal growth by regulating cytoskeletal remodeling and degradation of synaptic proteins.
Mutations in TRIM32 cause Limb-Girdle Muscular Dystrophy type 2H and Bardet-Biedl syndrome, disorders characterized by muscle weakness, retinal degeneration, and developmental defects. These mutations often impair E3 ligase activity or disrupt substrate binding, leading to accumulation of misfolded or damaged proteins. TRIM32 is also implicated in tumorigenesis, acting as either a tumor suppressor or oncogene depending on cellular context. Its ubiquitination of MYC and p53 pathway components links it directly to cancer cell proliferation and apoptosis.
Because TRIM32 integrates ubiquitin signaling with cellular differentiation and homeostasis, it serves as an essential node in protein quality control and tissue development. NSJ Bioreagents provides a validated TRIM32 antibody optimized for western blot, immunofluorescence, and ubiquitination assays, supporting research into muscle physiology, neurodegeneration, and cancer signaling. -
UniProt
- Q13049
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途径
- Negative Regulation of intrinsic apoptotic Signaling
抗原
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