PKN3 抗体 (AA 2-889)
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Quick Overview for PKN3 抗体 (AA 2-889) (ABIN7871827)
抗原
See all PKN3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 2-889
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原理
- PKN3 Antibody / Protein kinase N3
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human PKN3 recombinant protein (Position: E2-P889) was used as the immunogen for the PKN3 antibody.
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亚型
- IgG
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anti-Protein Kinase N3 (PKN3) (AA 1-252) antibody
PKN3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase N3 (PKN3) (AA 1-889) antibodyPKN3 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase N3 (PKN3) (AA 1-889) antibodyPKN3 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Protein Kinase N3 (PKN3) (Internal Region) antibodyPKN3 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase N3 (PKN3) (AA 225-275) antibodyPKN3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Protein Kinase N3 (PKN3) (C-Term) antibodyPKN3 适用: 人 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the PKN3 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the PKN3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- PKN3 (Protein Kinase N3 (PKN3))
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别名
- PKN3
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背景
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PKN3 antibody detects Protein kinase N3, a serine/threonine kinase involved in cytoskeletal organization, cell adhesion, and tumorigenesis. Encoded by the PKN3 gene on chromosome 9q31.2, this enzyme is a member of the protein kinase N (PKN) family, which are effectors of the Rho family of small GTPases. PKN3 links Rho signaling to downstream pathways that regulate actin dynamics, cell migration, and proliferation, making it a key mediator of cytoskeletal remodeling and oncogenic transformation.
Structurally, PKN3 contains an N-terminal regulatory region with HR1 (homology region 1) motifs that bind Rho GTPases, followed by a C-terminal catalytic domain similar to protein kinase C (PKC). PKN3 phosphorylates substrates involved in actin filament stabilization and cell junction assembly, coordinating mechanical and adhesive cues. It is localized to the cytoplasm and plasma membrane, where it interacts with focal adhesion proteins and scaffolds to transmit signals influencing motility and growth.
The PKN3 antibody is widely used in cancer biology, cell signaling, and cytoskeletal research to investigate Rho-dependent kinase pathways and cell morphology control. Western blot analysis detects a 110 kilodalton band corresponding to PKN3, while immunofluorescence shows diffuse cytoplasmic and membrane-associated staining in epithelial and tumor cells. This antibody supports research into the molecular basis of migration, invasion, and metastatic progression.
PKN3 is overexpressed in several cancers, including breast, prostate, and pancreatic carcinomas, where it promotes invasive behavior through activation of the PI3K/AKT and RhoA pathways. Genetic or pharmacologic inhibition of PKN3 suppresses tumor growth and metastasis, making it a promising therapeutic target. Beyond oncology, PKN3 also contributes to endothelial cell barrier function and angiogenesis. The PKN3 antibody provides a valuable reagent for dissecting the cellular functions and therapeutic potential of this kinase. NSJ Bioreagents validates this antibody for its applications, ensuring consistent and reliable results for signal transduction research. -
UniProt
- Q6P5Z2
抗原
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