MICAL-Like 1 抗体 (AA 192-747)
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Quick Overview for MICAL-Like 1 抗体 (AA 192-747) (ABIN7871677)
抗原
See all MICAL-Like 1 (MICALL1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 192-747
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原理
- MICALL1 Antibody / MICAL-like 1
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纯化方法
- Immunogen affinity purified
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免疫原
- E.coli-derived human MICALL1 recombinant protein (Position: R192-K747) was used as the immunogen for the MICALL1 antibody.
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亚型
- IgG
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anti-MICAL-Like 1 (MICALL1) (AA 1-863) antibody
MICALL1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-MICAL-Like 1 (MICALL1) (N-Term) antibodyMICALL1 适用: 人, 山羊 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-MICAL-Like 1 (MICALL1) antibodyMICALL1 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 25GB8275 unconjugated
anti-MICAL-Like 1 (MICALL1) (AA 752-781), (C-Term) antibodyMICALL1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB24168 unconjugated
anti-MICAL-Like 1 (MICALL1) (AA 204-253) antibodyMICALL1 适用: 人, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-MICAL-Like 1 (MICALL1) (N-Term) antibodyMICALL1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-MICAL-Like 1 (MICALL1) (AA 759-789), (C-Term) antibodyMICALL1 适用: 人 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the MICALL1 antibody should be determined by the researcher.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
- After reconstitution, the MICALL1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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- MICAL-Like 1 (MICALL1)
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别名
- MICALL1
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背景
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MICALL1 antibody detects MICAL-like protein 1, a cytoskeletal and membrane remodeling protein that regulates endocytic recycling and actin dynamics. MICALL1 belongs to the MICAL family of flavoprotein monooxygenases and adaptors that control trafficking of surface receptors and junctional complexes. Unlike enzymatically active MICALs, MICALL1 acts primarily as a scaffold, coordinating interactions between small GTPases and cytoskeletal components. The MICALL1 antibody is widely used to study vesicular trafficking, recycling endosomes, and cellular polarity.
MICALL1 is encoded by the MICALL1 gene located on human chromosome 22q13.1. The protein is approximately 94 kilodaltons and includes a calponin homology (CH) domain, a LIM domain, and multiple coiled-coil regions that mediate binding to Rab GTPases and the EHD family of endocytic regulators. MICALL1 localizes to tubular recycling endosomes, where it coordinates membrane curvature and receptor sorting.
The MICALL1 antibody detects a 94 kilodalton protein by western blot and shows punctate cytoplasmic staining in immunofluorescence. MICALL1 interacts with Rab8, Rab11, and EHD1, forming a complex that facilitates recycling of receptors such as transferrin and integrins to the plasma membrane. It also binds to actin regulatory proteins, linking vesicle trafficking to cytoskeletal rearrangements and cell morphology maintenance.
Disruption of MICALL1 expression impairs membrane recycling and causes accumulation of endosomal tubules. Mutations in MICALL1 are associated with autosomal recessive neurodevelopmental disorders characterized by ataxia and delayed myelination. In epithelial cells, MICALL1 participates in maintaining tight junction integrity and apical-basal polarity, processes essential for tissue organization.
Because of its central role in coordinating membrane transport, MICALL1 contributes to diverse biological pathways, from nutrient uptake to synaptic vesicle recycling. NSJ Bioreagents provides a validated MICALL1 antibody optimized for its applications, supporting research into vesicular dynamics, receptor trafficking, and cell polarity mechanisms. -
UniProt
- Q8N3F8
抗原
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